Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis.
Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis.
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血管生成素 1 抑制肥大细胞激活并预防过敏反应
DOI：
10.1371/journal.pone.0089148
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bai Y
中科院分区：
文献类型：
--
作者：
Yao JH;Cui M;Li MT;Liu YN;He QH;Xiao JJ;Bai Y
由于过敏性疾病的发病率和死亡率逐年上升，如何有效地预防和管理这些疾病已成为一个重要的问题。肥大细胞在变态反应性疾病中起着重要的调节作用。血管生成素1（Ang-I）通过抑制血管通透性、白细胞迁移和细胞因子产生而表现出抗炎特性。然而，Ang-1在肥大细胞活化和过敏性疾病中的功能尚不清楚。我们的研究结果表明，Ang-1通过抑制IκB磷酸化和NF-κB核转位，减少脂多糖（LPS）诱导的肥大细胞产生促炎细胞因子。Ang-1还通过降低细胞内钙离子水平，强烈抑制化合物48/80诱导和Fcε RI介导的肥大细胞脱颗粒。在小鼠体内慢病毒介导的Ang-1递送表现出减轻IgE依赖性被动皮肤过敏反应（PCA）的渗漏。此外，外源性Ang-1干预治疗防止了小鼠由化合物48/80诱导的肠系膜肥大细胞脱颗粒，减弱了促炎细胞因子的增加，减轻了肺损伤，并改善了过敏性休克中的存活率。我们的研究结果首次揭示了Ang-1在肥大细胞活化中的重要作用，并确定了Ang-1对过敏性疾病的治疗作用。
Since morbidity and mortality rates of anaphylaxis diseases have been increasing year by year, how to prevent and manage these diseases effectively has become an important issue. Mast cells play a central regulatory role in allergic diseases. Angiopoietin1 (Ang-1) exhibits anti-inflammatory properties by inhibiting vascular permeability, leukocyte migration and cytokine production. However, Ang-1's function in mast cell activation and anaphylaxis diseases is unknown. The results of our study suggest that Ang-1 decreased lipopolysaccharide (LPS)-induced pro-inflammatory cytokines production of mast cells by suppressing IκB phosphorylation and NF-κB nuclear translocation. Ang-1 also strongly inhibited compound 48/80 induced and FcεRI-mediated mast cells degranulation by decreasing intracellular calcium levels in vitro. In vivo lentivirus-mediated delivery of Ang-1 in mice exhibited alleviated leakage in IgE-dependent passive cutaneous anaphylaxis (PCA). Furthermore, exogenous Ang-1 intervention treatment prevented mice from compound 48/80-induced mesentery mast cell degranulation, attenuated increases in pro-inflammatory cytokines, relieved lung injury, and improved survival in anaphylaxis shock. The results of our study reveal, for the first time, the important role of Ang-1 in the activation of mast cells, and identify a therapeutic effect of Ang-1 on anaphylaxis diseases.
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影响因子：
14.2
作者：
Ashmole, Ian;Duffy, S. Mark;Leyland, Mark L.;Morrison, Valerie S.;Begg, Malcolm;Bradding, Peter
通讯作者：
Bradding, Peter
影响因子：
4.8
作者：
Carlson, TR;Feng, YZ;Morla, AO
通讯作者：
Morla, AO
DOI：
10.1111/j.1476-5381.1951.tb00661.x
发表时间：
1951-01-01
期刊：
BRITISH JOURNAL OF PHARMACOLOGY AND CHEMOTHERAPY
影响因子：
--
作者：
PATON, WDM
通讯作者：
PATON, WDM
乳腺癌细胞中多梳蛋白 EZH2 整合雌激素和 Wnt 信号通路
影响因子：
5.3
作者：
Shi, Bin;Liang, Jing;Shang, Yongfeng
通讯作者：
Shang, Yongfeng
影响因子：
8.3
作者：
Ahmad, Shakil;Cudmore, Melissa J.;Ahmed, Asif
通讯作者：
Ahmed, Asif