A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein
A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein
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DOI：
10.1006/geno.1998.5672
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发表时间：
1999-02-15
期刊：
影响因子：
4.4
通讯作者：
Lomax, MI
中科院分区：
文献类型：
--
作者：
Adler, HJ;Winnicki, RS;Lomax, MI
鸡WDR 1基因表达在更高的水平在鸡的基底乳头后声过度刺激。3.3 kb的WDR 1 cDNA编码一种新的67 kDa的蛋白质，含有9个WD 40重复序列，介导蛋白质-蛋白质相互作用的基序。预测的WDR 1蛋白具有很高的序列同一性WD 40-重复蛋白在芽殖酵母（酿酒酵母），两种黏菌（盘状网柄菌和多头绒泡菌），和蛔虫（秀丽隐杆线虫）。酵母和多头绒泡菌蛋白结合肌动蛋白，表明新的鸡蛋白可能是肌动蛋白结合蛋白。序列数据库比较鉴定了与鸡WDR 1 cDNA具有高度序列同一性的小鼠和人cDNA。小鼠Wdr 1和人WDR 1蛋白显示95%的序列相同性彼此和86%的同一性，鸡WDR 1蛋白。噪音损伤后鸡基底乳头的总RNA的北方印迹分析显示，在损伤区域的3.1 kb的转录水平增加。WDR 1基因定位于人类4号染色体，距离端粒4p的22和24 cM之间。(C)北京：科学出版社.
The chick WDR1 gene is expressed at higher levels in the chick basilar papilla after acoustic overstimulation. The 3.3-kb WDR1 cDNA encodes a novel 67-kDa protein containing nine WD40 repeats, motifs that mediate protein-protein interactions. The predicted WDR1 protein has high sequence identity to WD40-repeat proteins in budding yeast (Saccharomyces cerevisiae), two slime molds (Dictyostelium discoideum and Physarum polycephalum), and the roundworm (Caenorhabditis elegans). The yeast and P. polycephalum proteins bind actin, suggesting that the novel chick protein may be an actin-binding protein. Sequence database comparisons identified mouse and human cDNAs with high sequence identity to the chick WDR1 cDNA. The mouse Wdr1 and human WDR1 proteins showed 95% sequence identity to each other and 86% identity to the chick WDR1 protein. Northern blot analysis of total RNA from the chick basilar papilla after noise trauma revealed increased levels of a 3.1-kb transcript in the lesioned area. The WDR1 gene was mapped to human chromosome 4, between 22 and 24 cM from the telomere of 4p. (C) 1999 Academic Press.