PDMS-based micro PCR chip with parylene coating
PDMS-based micro PCR chip with parylene coating
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DOI：
10.1088/0960-1317/13/5/332
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发表时间：
2003-09-01
影响因子：
2.3
通讯作者：
Chang, JK
中科院分区：
文献类型：
--
作者：
Shin, YS;Cho, K;Chang, JK
我们研制了一种用于聚合酶链反应（PCR）的聚二甲基硅氧烷（PDMS）微芯片。聚二甲基硅氧烷具有价格低廉、透明、易于制备和生物相容性好等优点。但在微PCR中，PDMS的多孔性会导致气泡形成、样品蒸发和蛋白质吸附等关键问题。为了解决这些问题，我们用Parylene薄膜涂覆微型PCR芯片，它具有低透湿性和长期稳定性。通过数值分析研究了PDMS和Parylene的低导热系数对PCR芯片热特性的影响。比较了硅、玻璃和PDMS三种材料的微型PCR芯片的热响应。根据结果，我们通过加热顶侧和底侧来确定基于PDMS的微型PCR芯片的适当热响应。我们可以成功地扩增血管紧张素转换酶基因的小体积为2穆尔的PDMS为基础的微型PCR芯片上没有任何添加剂。
We have developed a microchip for polymerase chain reaction (PCR) with polydimethylsiloxane (PDMS). PDMS has good characteristics: it is cheap, transparent, easy to fabricate and biocompatible. But in micro PCR, the porosity of PDMS causes several critical problems such as bubble formation, sample evaporation and protein adsorption. To solve those problems, we coated the micro PCR chips with Parylene film, which has low permeability to moisture and long-term stability. We investigated the influence of low thermal conductivity of PDMS and Parylene on the thermal characteristics of the PCR chips with numerical analysis. The thermal responses of micro PCR chips were compared for three materials: silicon, glass and PDMS. From the results, we identified appropriate thermal responses of the PDMS-based micro PCR chips by heating both the top and bottom sides. We could successfully amplify the angiotensin converting enzyme gene with as small a volume as 2 mul on the PDMS-based micro PCR chips without any additives.