CLONAL DISPERSION IN PROLIFERATIVE LAYERS OF DEVELOPING CEREBRAL-CORTEX
CLONAL DISPERSION IN PROLIFERATIVE LAYERS OF DEVELOPING CEREBRAL-CORTEX
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DOI：
10.1038/362632a0
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发表时间：
1993-04-15
期刊：
影响因子：
64.8
通讯作者：
CEPKO, CL
中科院分区：
文献类型：
--
作者：
WALSH, C;CEPKO, CL
在成人大脑皮层中，许多逆转录病毒标记的克隆广泛分布1，尽管这种分散的机制还不清楚。在这里，我们调查的克隆分散的时间序列标记后的大鼠皮质细胞的祖细胞与复制能力低下的逆转录病毒在皮质神经发生的早期阶段，14-15天后的概念（E14/15）。在3、6或10天后确定标记的子细胞的位置。感染后3天（E18），标记的同胞细胞呈放射状排列。相比之下，感染后6天（E20/21），43%的皮质克隆分散非径向至少500妈妈。四个这些广泛的克隆分散纵向大于或等于2毫米，这意味着持续的分散率> 15微米每小时。分散的兄弟细胞发生在增殖区的前脑中的35%的广泛分散的克隆，这表明一些分散反映运动的分裂细胞。一些克隆扩散到新皮层以外的嗅球中。祖细胞分散代表了一种以前未被认识到的迁移模式，通过这种模式，兄弟细胞在发育中的前脑中广泛分散。
IN the adult cerebral cortex, many retrovirally labelled clones are widely dispersed1, though the mechanisms of this dispersion are not well understood. Here we investigate the temporal sequence of clonal dispersion after labelling progenitors of rat cortical cells with replication-incompetent retroviruses at early stages of cortical neurogenesis, 14-15 days after conception (E14/15). The location of labelled daughter cells was determined 3, 6 or 10 days later. Labelled sibling cells were radially arrayed three days after infection (E18). In contrast, by six days after infection (E20/21), 43% of cortical clones were dispersed non-radially by at least 500 mum. Four of these widespread clones were dispersed longitudinally by greater-than-or-equal-to 2 mm, implying sustained rates of dispersion of > 15 mum per hour. Dispersed sibling cells occurred within proliferative zones of the forebrain in 35% of widely dispersed clones, suggesting that some dispersion reflects movement of dividing cells. Some clones dispersed beyond the neocortex into the olfactory bulb. Progenitor cell dispersion represents a previously unrecognized mode of migration by which sibling cells become widely dispersed in the developing forebrain.