Human Hepatic UGT2B15 Developmental Expression
Human Hepatic UGT2B15 Developmental Expression
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DOI：
10.1093/toxsci/kfu126
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发表时间：
2014-09-01
影响因子：
3.8
通讯作者：
McCarver, D. Gail
中科院分区：
文献类型：
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作者：
Divakaran, Karthika;Hines, Ronald N.;McCarver, D. Gail
人肝脏UGT 2B 15发育表达变化可能会改变重要药物和毒物（如双酚A（BPA））的代谢。以前，UGT 2B 15个体发育知识由转录数据组成，这是蛋白质表达的可疑替代物。在此，测定了人肝微粒体（n=236，妊娠8周至18岁）中的UGT 2B 15蛋白含量。还检测了UGT 2B 15 *2和 *5等位基因中常见的功能性单核苷酸多态性（g.253G>T）的影响。UGT 2B 15表达始于胎儿晚期，约为成熟值的18%（中位数分别为48、267 pmole/mg微粒体蛋白; p= 28周，n=10），含量相似，但低于3 - 15周龄婴儿（n=46;中位数分别为38、48、404 pmole/mg微粒体蛋白; p = 10）。
Human hepatic UGT2B15 developmental expression changes may alter the metabolism of important drugs and toxicants such as bisphenol A (BPA). Previously, UGT2B15 ontogeny knowledge consisted of transcript data, a dubious surrogate for protein expression. Herein, UGT2B15 protein content was determined in human hepatic microsomes (n=236, 8 weeks gestation to 18 years). The impact of a common, functional single nucleotide polymorphism (g.253G>T),present in UGT2B15*2 and *5 alleles, was also tested. UGT2B15 expression began during late fetal life, at about 18% of mature values (medians=48, 267 pmoles/mg of microsomal protein, respectively; p= 28 weeks, n=10) content was similar, but lower than that of infants between 3 and 15 weeks age (n=46; medians=38, 48, 404 pmoles/mg microsomal protein, respectively; p