Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae
Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae
复制标题
酿酒酵母盐胁迫反应中溴结构域因子1的遗传和比较转录组分析
DOI：
10.1007/s00284-006-0525-4
复制
发表时间：
2007-04-01
影响因子：
2.6
通讯作者：
Bao, Xiaoming
中科院分区：
文献类型：
--
作者：
Liu, Xiangyong;Zhang, Xiaohua;Bao, Xiaoming
酿酒酵母BDF 1基因，它编码一个含溴结构域的转录因子，先前分离的转座子在盐敏感性突变体的屏幕中的突变。然而，溴结构域转录因子1蛋白（Bdf 1 p）调控的盐胁迫反应机制仍然知之甚少。遗传分析表明，BDF 1缺失突变体的盐敏感性受到其同源基因BDF 2基因剂量的抑制。此外，比较转录组分析显示，在盐胁迫（0.6 mol/L NaCl，45 min）的存在下，野生型和bdf 1 Delta突变体之间的转录反应的差异主要与细胞壁的生物合成，线粒体，和一些未知的基因。我们的研究结果提供了有关盐胁迫反应的调控机制的进一步信息，并为了解含溴结构域蛋白在细胞过程中的生物学功能提供了新的见解。
The Saccharomyces cerevisiae BDF1 gene, which encodes a bromodomain-containing transcription factor, was previously isolated by transposon mutugenesis in a screen for salt-sensitive mutants. However, the salt stress response mechanism regulated by bromodomain transcription factor 1 protein (Bdf1p) remains poorly understood. In this report, genetic analysis indicated that the salt sensitivity of the BDF1 deletion mutant was suppressed by increased gene dosage of its homologous gene BDF2. Furthermore, comparative transcriptome analysis revealed that the differences in transcriptional response between the wild type and the bdf1 Delta mutant in the presence of salt stress (0.6 mol/L NaCl, 45 min) were mainly related to cell-wall biosynthesis, the mitochondria, and several unknown genes. Our results provided further information about the regulatory mechanism involved in the salt stress response and adds new insight for understanding the biological functional of bromdomain-containing proteins in cellular processes.