Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites.
Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites.
复制标题
小鼠巨噬细胞系中的人皮肤利什曼病：细胞内寄生虫的繁殖和分离。
作者：
K. Chang
小鼠巨噬细胞系 J774G8 支持墨西哥亚马逊利什曼原虫（南美皮肤利什曼病的病原体）在细胞内持续大量生长。通过简单的 Percoll 梯度离心，可以以高回收率和纯度从这些受感染的培养物中分离出细胞内寄生虫。
A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.