Distribution of streptococcal inhibitor of complement variants in pharyngitis and invasive isolates in an epidemic of serotype M1 group a Streptococcus infection
Distribution of streptococcal inhibitor of complement variants in pharyngitis and invasive isolates in an epidemic of serotype M1 group a Streptococcus infection
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DOI：
10.1086/318543
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发表时间：
2001-02-15
影响因子：
6.4
通讯作者：
Musser, JM
中科院分区：
文献类型：
--
作者：
Hoe, NP;Vuopio-Varkila, J;Musser, JM
补体链球菌抑制因子(SIC)是一种高度多态的胞外蛋白，主要由M1型A组链球菌(GAS)产生。由于积极的自然选择，新的SIC蛋白变种经常出现在M1流行病中。为了进一步了解M1流行的分子基础，在一项为期37个月的基于人群的监测研究中，对居住在芬兰赫尔辛基的598名患者的471例咽炎和127株化脓性和血液分离株进行了SIC基因测序。从化脓性感染和血液中恢复的大多数M1毒气亚克隆在引起咽炎的亚克隆池中大量存在。咽炎、化脓性和血液样本之间共有的等位基因在咽部分离株中被鉴定出来，平均9.8个月后它们从化脓性感染和血液中恢复，这表明大多数SIC变异体的选择发生在粘膜表面。相反，在EMM和COVR/COVS基因中没有发现变异。
Streptococcal inhibitor of complement (Sic) is a highly polymorphic extracellular protein made predominantly by serotype M1 group A Streptococcus (GAS). New variants of the Sic protein frequently appear in M1 epidemics as a result of positive natural selection. To gain further understanding of the molecular basis of M1 epidemics, the sic gene was sequenced from 471 pharyngitis and 127 pyogenic and blood isolates recovered from 598 patients living in metropolitan Helsinki, Finland, during a 37-month population-based surveillance study. Most M1 GAS subclones recovered from pyogenic infections and blood were abundantly represented in the pool of subclones causing pharyngitis. Alleles shared among the pharyngitis, pyogenic, and blood samples were identified in throat isolates a mean of 9.8 months before their recovery from pyogenic infections and blood, which indicates that selection of most sic variants occurs on mucosal surfaces. In contrast, no variation was identified in the emm and covR/covS genes.