THIOL GROUPS OF NORMAL ADULT HUMAN HAEMOGLOBIN
THIOL GROUPS OF NORMAL ADULT HUMAN HAEMOGLOBIN
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DOI：
10.1042/bj0690027
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发表时间：
1958-01-01
影响因子：
4.1
通讯作者：
CECIL, R
中科院分区：
文献类型：
--
作者：
ALLISON, AC;CECIL, R
鉴于早期工作中的差异，重新研究了正常成人血红蛋白的巯基含量。用氯化汞和氢氧化苯汞对天然蛋白和变性蛋白进行电流滴定。天然氢氧化碳氧血红蛋白。天然碳氧血红蛋白结合2.2个Hg++离子和2.2个苯汞氢氧化物分子/分子。给出了得出所形成的2种硫醇盐为Prot：S：HgCl和Prot：S：HgPh的结论的理由。因此，2.2的数字代表天然蛋白质的反应性巯基/分子，而先前的工作者给出的是4。变性碳氧血红蛋白结合三个Hg++离子和6个苯汞氢氧化物分子/分子。在这种情况下，形成的2个硫醇盐被认为是（Prot：S）2 Hg和Prot：S：HgPh。因此，巯基总数/分子的羧基血红蛋白是6，而以前的工作者给出的是8。
In view of discrepancies in earlier work the thiol content of normal adult human hemoglobin was reinvestigated. Both the native and denatured protein were titrated amperometrically with mercuric chloride and with phenylmercuric hydroxide. Native carboxyhemoglobin hydroxide. Native carboxyhemoglobin binds 2.2 Hg++ions and 2.2 molecules of phenylmercuric hydroxide/molecule. Reasons are given for concluding that the 2 mercaptides formed are Prot:S:HgCl and Prot:S:HgPh. The figure of 2.2 therefore represents the reactive thiol groups/molecule of native protein, as compared with 4 given by previous workers. Denatured carboxyhaemoglobin binds three Hg++ ions and 6 molecules of phenylmercuric hydroxide/molecule. In this case the 2 mercaptides formed are assumed to be (Prot:S)2Hg and Prot:S:HgPh. The total number of thiol groups/molecule of carboxyhaemoglobin is therefore 6, as compared with 8 given by previous workers.