The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae
The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae
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DOI：
10.1128/mcb.21.7.2449-2462.2001
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发表时间：
2001-04-01
影响因子：
5.3
通讯作者：
Snyder, M
中科院分区：
文献类型：
--
作者：
Bidlingmaier, S;Weiss, EL;Snyder, M
在萌芽的早期阶段，细胞小重塑和极化分泌物集中在芽尖（顶端生长）。CBK 1基因，编码一个假定的丝氨酸/苏氨酸蛋白激酶，被确定在一个屏幕上设计，以隔离突变，影响顶端生长。Cbk 1 Delta细胞的分析表明，Cbk 1 p是必需的有效的顶端生长，适当的交配投影形态，双极芽位点选择二倍体细胞，和细胞分离。表位标记的Cbk 1 p定位于两侧的芽颈在后期，细胞分离之前。CBK 1和另一个基因HYM 1先前在筛选参与转录抑制的基因时被鉴定，并被认为在相同的途径中起作用。HYM 1的缺失导致的表型与在cbk 1 Delta细胞中观察到的表型相似，并破坏了Cbk 1 p的芽颈定位。cbk 1 Delta的全基因组转录分析表明，激酶调节许多基因的表达，vith细胞mall相关的功能，包括两个基因所需的有效的细胞分离：几丁质酶编码基因CTS 1和葡聚糖酶编码基因SCW 11。Ace 2 p转录因子是CTS 1表达所需的，并且已经显示与Cbklp物理相互作用。ace 2 δ细胞的分析揭示Ace 2 p是细胞分离所需的，但不是极化生长所需的。我们的研究结果表明，Cbk 1 p和Hym 1 p的功能，以调节两个不同的细胞形态发生途径：一个ACE 2独立的途径，这是需要有效的顶端生长和交配突起的形成和ACE 2依赖的途径，这是需要有效的细胞分离后胞质分裂。Cbk 1 p与粗糙脉孢菌Cot-1、粟酒裂殖酵母Orb 6、秀丽隐杆线虫、果蝇和人类Ndr以及果蝇和哺乳动物WARTS/LATS激酶最密切相关。许多Cbk 1相关激酶已被证明可以调节细胞形态。
During the early stages of budding, cell mall remodeling and polarized secretion are concentrated at the bud tip (apical growth). The CBK1 gene, encoding a putative serine/threonine protein kinase, was identified in a screen designed to isolate mutations that affect apical growth. Analysis of cbk1 Delta cells reveals that Cbk1p is required for efficient apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation. Epitope-tagged Cbk1p localizes to both sides of the bud neck in late anaphase, just prior to cell separation. CBK1 and another gene, HYM1, were previously identified in a screen for genes involved in transcriptional repression and proposed to function in the same pathway. Deletion of HYM1 causes phenotypes similar to those observed in cbk1 Delta cells and disrupts the bud neck localization of Cbk1p. Whole-genome transcriptional analysis of cbk1 Delta suggests that the kinase regulates the expression of a number of genes,vith cell mall-related functions, including two genes required for efficient cell separation: the chitinase-encoding gene CTS1 and the glucanase-encoding gene SCW11. The Ace2p transcription factor is required for expression of CTS1 and has been shown to physically interact,vith Cbklp, Analysis of ace2 Delta cells reveals that Ace2p is required for cell separation but not for polarized growth. Our results suggest that Cbk1p and Hym1p function to regulate two distinct cell morphogenesis pathways: an ACE2-independent pathway that is required for efficient apical growth and mating projection formation and an ACE2-dependent pathway that is required for efficient cell separation following cytokinesis. Cbk1p is most closely related to the Neurospora crassa Cot-1; Schizosaccharomyces pombe Orb6; Caenorhabditis elegans, Drosophila, and human Ndr; and Drosophila and mammalian WARTS/LATS kinases. Many Cbk1-related kinases have been shown to regulate cellular morphology.