Subcellular localization of plasmids containing the oriC region of the Escherichia coli chromosome, with or without the sopABC partitioning system
Subcellular localization of plasmids containing the oriC region of the Escherichia coli chromosome, with or without the sopABC partitioning system
复制标题
DOI：
10.1046/j.1365-2958.1999.01611.x
复制
发表时间：
1999-11-01
影响因子：
3.6
通讯作者：
Hiraga, S
中科院分区：
文献类型：
--
作者：
Niki, H;Hiraga, S
荧光原位杂交（FISH）分析揭示了大肠杆菌细胞分裂周期中特定染色体片段和质粒分子的亚细胞定位：染色体上的复制起点（oriC）片段定位于类核边界，而主动分配的mini-F质粒分子定位于细胞的1/4和3/4位置。相反，缺乏sopABC片段的迷你F质粒分子随机定位在细胞两极的细胞质区域。在这项研究中，我们分析了一个oriC质粒的亚细胞定位，含有最小的E。coli染色体复制起点及其侧翼区。这些oriC质粒分子主要定位于细胞两极的胞质区域。另一方面，携带F质粒sopABC片段的oriC质粒DNA分子定位于细胞区位点，如积极分区mini-F质粒DNA分子。因此，我们得出结论，oriC本身及其侧翼区不足以定位E.大肠杆菌的染色体。
Fluorescence in situ hybridization (FISH) analysis has revealed the subcellular localization of specific chromosomal segments and plasmid molecules during the cell division cycle in Escherichia coli: the replication origin (oriC) segments on the chromosome are localized at nucleoid borders, and actively partitioning mini-F plasmid molecules are localized at the 1/4 and 3/4 positions of the cell. In contrast, mini-F plasmid molecules lacking the sopABC segment are randomly localized in cytoplasmic areas at cell poles. In this study, we analysed the subcellular localization of an oriC plasmid that contains the minimum E. coli chromosomal replication origin and its flanking regions. These oriC plasmid molecules were mainly localized in cytosolic areas at cell poles. On the other hand, oriC plasmid DNA molecules carrying the sopABC segment of F plasmid were localized at cell quarter sites, as were actively partitioning mini-F plasmid DNA molecules. Therefore, we conclude that oriC itself and its flanking regions are not sufficient for positioning the replication origin domain of the E. coli chromosome within the cell.