Analysis of Differential Gene Expression and Core Canonical Pathways Involved in the Epithelial to Mesenchymal Transition of Triple Negative Breast Cancer Cells by Ingenuity Pathway Analysis.
Analysis of Differential Gene Expression and Core Canonical Pathways Involved in the Epithelial to Mesenchymal Transition of Triple Negative Breast Cancer Cells by Ingenuity Pathway Analysis.
复制标题
DOI：
10.4236/cmb.2023.132002
复制
发表时间：
2023-06
期刊：
影响因子：
--
通讯作者：
Banerjee H
中科院分区：
文献类型：
--
作者：
Cagle E;Lake B;Banerjee A;Cuffee J;Banerjee N;Gilmartin D;Liverman M;Brown S;Armstrong E;Bhattacharya S;Ghosh S;Mandal T;Banerjee H
三阴性乳腺癌(TNBC)是一种死亡率和发病率都很高的恶性肿瘤。上皮向间充质转化(EMT)是上皮源性癌细胞中最常见的病理生理变化，可促进肿瘤的转移、耐药和肿瘤干细胞的形成。由于有关TNBC细胞差异基因表达和导致EMT的细胞信号事件的信息有限，本研究通过比较EMT模型TNBC细胞株和正常TNBC细胞株的RNA分离和测序所产生的转录数据来完成这项研究。转录数据的RNA测序和独创性路径软件分析(IPA)揭示了几个上调和下调的基因表达，以及参与TNBC细胞EMT变化的新的核心典型途径，包括Sirtuin信号、氧化磷酸化和线粒体功能障碍事件。
Triple Negative Breast Cancer (TNBC) is a malignant form of cancer with very high mortality and morbidity. Epithelial to Mesenchymal Transition (EMT) is the most common pathophysiological change observed in cancer cells of epithelial origin that promotes metastasis, drug resistance and cancer stem cell formation. Since the information regarding differential gene expression in TNBC cells and cell signaling events leading to EMT is limited, this investigation was done by comparing transcriptomic data generated by RNA isolation and sequencing of a EMT model TNBC cell line in comparison to regular TNBC cells. RNA sequencing and Ingenuity Pathway Software Analysis (IPA) of the transcriptomic data revealed several upregulated and downregulated gene expressions along with novel core canonical pathways including Sirtuin signaling, Oxidative Phosphorylation and Mitochondrial dysfunction events involved in EMT changes of the TNBC cells.