Heymann antigen GP330 demonstrates affinity for fibronectin, laminin, and type I collagen and mediates rat proximal tubule epithelial cell adherence to such matrices in vitro.
Heymann antigen GP330 demonstrates affinity for fibronectin, laminin, and type I collagen and mediates rat proximal tubule epithelial cell adherence to such matrices in vitro.
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Heymann 抗原 GP330 表现出对纤连蛋白、层粘连蛋白和 I 型胶原的亲和力，并在体外介导大鼠近端小管上皮细胞与此类基质的粘附。
DOI：
10.1016/0014-4827(90)90273-d
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发表时间：
1990
影响因子：
3.7
通讯作者：
Rennke,HG
中科院分区：
文献类型：
--
作者：
Mendrick,DL;Chung,DC;Rennke,HG
我们已经利用单克隆抗体直接对糖蛋白的表面上的近端小管上皮细胞（PTEC），以研究其与基质成分的相互作用。暴露于针对330 kDa细胞表面糖蛋白的单克隆抗体的PTEC表现出对I型胶原蛋白、纤连蛋白、层粘连蛋白和明胶包被的组织培养表面的附着和增殖的显著表位特异性抑制。这种效应不是由于抗体毒性，因为这些细胞在悬浮培养物中没有表现出代谢功能障碍，并且在从细胞表面去除抗体后，抑制作用可以逆转。此外，洗涤剂溶解的gp 330表现出特定的亲和力，纤连蛋白，层粘连蛋白，和I型胶原蛋白，这是不受抑制的精氨酸-甘氨酸-天冬氨酸含肽。针对受体表位的单克隆抗体能够促进PTEC粘附和生长时，这种抗体被固定在细胞培养皿。尽管gp 330在新鲜分离的PTEC的原代培养物中充当基质蛋白的受体，但这种作用在已建立的培养物中并不明显。这些结果表明，新鲜分离的PTEC依赖于gp 330附着到基质分子上，而体外适应的PTEC依赖于培养条件下激活的其他受体。gp 330对基质分子的亲和力可能与实验性膜性肾病（Heymann肾炎）中肾小球毛细血管壁中形成的含gp 330的免疫复合物的持久性有关。
We have utilized monoclonal antibodies directed against glycoproteins on the surface of proximal tubule epithelial cells (PTEC) to study their interaction with matrix components. PTEC exposed to monoclonal antibodies directed against a 330-kDa cell surface glycoprotein exhibited a significant epitope-specific inhibition of attachment and proliferation on type I collagen-, fibronectin-, laminin-, and gelatin-coated tissue culture surfaces. This effect was not due to antibody toxicity since such cells did not exhibit metabolic dysfunction in suspension cultures and the inhibition could be reversed upon removal of the antibody from the cell surface. Furthermore, detergent-solubilized gp330 demonstrated specific affinity for fibronectin, laminin, and type I collagen which was not inhibited by ArgGlyAsp-containing peptides. A monoclonal antibody directed against the receptor epitope was capable of promoting PTEC adherence and growth when such an antibody was immobilized on cell culture dishes. Although gp330 acted as a receptor for matrix proteins in primary cultures of freshly isolated PTEC, this effect was not demonstrable in established cultures. These results suggest that freshly isolated PTEC depend on gp330 for their attachment to matrix molecules whilein vitro-adapted PTEC rely on other receptors activated by culture conditions. The affinity of gp330 for matrix molecules may be of pathogenic relevance in the persistence of gp330-containing immune complexes formed in the glomerular capillary wall in experimental membranous nephropathy (Heymann nephritis).
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DOI：
--
发表时间：
2006
期刊：
総務省統計研修所 リサーチペーパー 第5号
影响因子：
--
作者：
元山 斉;山口 幸三;ryoko Morozumi;美添泰人・荒木万寿夫
通讯作者：
美添泰人・荒木万寿夫
淋巴前体细胞粘附在纤连蛋白上的两个不同位点
影响因子：
7.8
作者：
P. Bernardi;V. Patel;H. Lodish
通讯作者：
H. Lodish
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影响因子：
19.6
作者：
Mendrick,DL;Rennke,HG
通讯作者：
Rennke,HG
单克隆抗体表位特异性诱导蛋白尿。
影响因子：
19.6
作者：
Mendrick,DL;Rennke,HG
通讯作者：
Rennke,HG
影响因子：
4.4
作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
通讯作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer