REGULATION OF INTERLEUKIN-8 GENE-EXPRESSION IN HUMAN ENDOMETRIAL CELLS IN CULTURE
REGULATION OF INTERLEUKIN-8 GENE-EXPRESSION IN HUMAN ENDOMETRIAL CELLS IN CULTURE
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DOI：
10.1016/0303-7207(93)90168-j
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发表时间：
1993-08-01
影响因子：
4.1
通讯作者：
CASEY, ML
中科院分区：
文献类型：
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作者：
ARICI, A;HEAD, JR;CASEY, ML
在这项研究中，我们研究了白细胞介素-8（IL-8）基因在人子宫内膜间质细胞和上皮细胞中的表达调控。这项研究是作为这些细胞在调节白细胞向子宫内膜募集中的作用分析的一部分进行的。良好的特征模型系统被用来研究子宫内膜IL-8基因表达的调节，即，在单层培养的基质细胞后，第一次传代和腺上皮细胞在原代培养。用IL-1 α、肿瘤坏死因子-α或血清处理子宫内膜间质细胞后，培养液中IL-8 mRNA的水平和免疫反应性IL-8的积累以时间和浓度依赖性方式增加。IL-1 α加血清对IL-8 mRNA水平的影响至少是相加的。血清治疗引起子宫内膜间质细胞中IL-8基因转录的适度刺激（治疗6小时后评估），但血清也在这些间质细胞中起作用，使IL-8 mRNA的半衰期延长2.5倍以上。子宫内膜上皮细胞IL-8 mRNA水平的调节与间质细胞明显不同。首先，在无血清培养基中未处理的上皮细胞中IL-8 mRNA的水平远大于在类似条件下基质细胞中的水平。第二，而子宫内膜上皮细胞中的IL-8 mRNA水平也增加血清和IL-1在血清的情况下，在血清的存在下，IL-1治疗引起的IL-8 mRNA水平没有明显的变化，是在子宫内膜间质细胞的情况。
In this study, we investigated the regulation of interleukin-8 (IL-8) gene expression in separated endometrial stromal and epithelial cells of human endometrium. This research was conducted as part of an analysis of the role of these cells in regulating the recruitment of leukocYtes to the endometrium. Well-characterized model systems were used to study the regulation of endometrial IL-8 gene expression, namely, stromal cells in monolayer culture after first passage and glandular epithelium in primary culture. The levels of IL-8 mRNA and the accumulation of immunoreactive IL-8 in the medium of endometrial stromal cells is culture increased in a time- and concentration-dependent manner upon treatment with IL-1alpha, tumor necrosis factor-alpha ,or serum. The effects of IL-1alpha plus serum on IL-8 mRNA levels were at least additive. Serum treatment caused a modest stimulation of IL-8 gene transcription (evaluated after 6 h of treatment) in endometrial stromal cells, but serum also acted in these stromal cells to prolong the half-life of IL-8 mRNA by more than 2.5-fold. The regulation of the levels of IL-8 mRNA in endometrial epithelial cells is distinctly different from that in stroma. First, the levels of IL-8 mRNA in non-treated epithelial cells in serum-free medium were much greater than those in stromal cells under similar conditions. Second, whereas the levels of IL-8 mRNA in endometrial epithelial cells also increased in response to serum and to IL-1 in the absence of serum, in the presence of serum, IL-1 treatment caused no appreciable change in the levels of IL-8 mRNA as was the case in endometrial stromal cells.