Untargeted metabolomics analysis of the anti-diabetic effect of Red ginseng extract in Type 2 diabetes Mellitus rats based on UHPLC-MS/MS
Untargeted metabolomics analysis of the anti-diabetic effect of Red ginseng extract in Type 2 diabetes Mellitus rats based on UHPLC-MS/MS
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DOI：
10.1016/j.biopha.2021.112495
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发表时间：
2021-12-07
影响因子：
7.5
通讯作者：
Wu, Xiaohui
中科院分区：
文献类型：
--
作者：
Yang, Zijun;Wang, Dan;Wu, Xiaohui
红参是一种传统的中草药，长期以来一直用于治疗糖尿病，其降血糖活性已被证实。然而，红参在代谢水平上对2型糖尿病（T2 DM）的作用机制尚不清楚。本研究旨在从非靶向代谢组学角度探讨红参提取物对2型糖尿病大鼠的治疗作用。采用高脂饮食（HFD）联合链脲佐菌素（STZ）诱导T2 DM大鼠模型，治疗4周后采集血清标本。采用超高效液相色谱-Q Exactive HF-X质谱联用仪分析血清中代谢物水平，评价各组间代谢水平的差异。生化分析结果显示，红参提取物干预4周后显著改善了总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、血糖（GLU）和空腹胰岛素（FINS）水平。采用正交偏最小二乘判别分析法研究大鼠代谢组学的总体变化。红参提取物干预后，50项生物标志物呈回调趋势。代谢途径富集分析表明，调控途径为D-精氨酸和D-鸟氨酸代谢、D-谷氨酰胺和D-谷氨酸代谢、牛磺酸和亚牛磺酸代谢、精氨酸生物合成和色氨酸代谢。结果表明，红参提取物对T2 DM有一定的治疗作用，其机制可能与改善代谢紊乱有关。
Red ginseng is a traditional Chinese herbal medicine that has long been used to treat diabetes, and its blood sugar-lowering activity has been confirmed. However, the mechanism of action of red ginseng on type 2 diabetes mellitus (T2DM) at the metabolic level is still unclear. The purpose of this study is to investigate the effect of red ginseng extract in the treatment of T2DM rats based on untargeted metabolomics. The rat model of T2DM was induced by a high-fat diet (HFD) combined with streptozotocin (STZ), and serum samples were collected after four weeks of treatment. The ultra-high-performance liquid chromatography coupled with Q Exactive HF-X Mass Spectrometer was used to analyze the level of metabolites in serum to evaluate the differences in metabolic levels between different groups. The results of biochemical analysis showed that red ginseng extract intervention significantly improved the levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), serum glucose (GLU), and fasting insulin (FINS) after four weeks. Orthogonal partial least squares discriminant analysis was used to study the overall changes of rat metabolomics. After the intervention of red ginseng extract, 50 biomarkers showed a callback trend. Metabolic pathway enrichment analysis showed that the regulated pathways were D-arginine and D-ornithine metabolism, D-glutamine and D-glutamate metabolism, taurine and hypotaurine metabolism, arginine biosynthesis, and tryptophan metabolism. Generally, the results demonstrated that red ginseng extract had beneficial effects on T2DM, which could be mediated via ameliorating the metabolic disorders.