A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts
A pentatricopeptide repeat protein is essential for RNA editing in chloroplasts
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DOI：
10.1038/nature03229
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发表时间：
2005-01-20
期刊：
影响因子：
64.8
通讯作者：
Shikanai, T
中科院分区：
文献类型：
--
作者：
Kotera, E;Tasaka, M;Shikanai, T
RNA 编辑是涉及核苷酸插入、删除或修饰的 RNA 成熟过程(1)。在高等植物的细胞器转录物中，特定的胞苷残基转化为尿苷残基。在许多情况下，编辑会导致保守氨基酸残基的恢复，这一过程对于质体中的蛋白质功能至关重要(2,3)。尽管在建立用于分析 RNA 编辑的体内 (4) 和体外 (5) 系统方面取得了技术突破，但其在高等植物中的机制仍有待鉴定。在这里，我们介绍了一种遗传方法，并报告了负责叶绿体中特定 RNA 编辑事件的基因的发现。
RNA editing is a process of RNA maturation involved in the insertion, deletion or modification of nucleotides(1). In organellar transcripts of higher plants, specific cytidine residues are converted into uridine residues. In many cases, editing results in the restoration of conserved amino acid residues, a process that is essential for protein function in plastids(2,3). Despite the technical breakthrough in establishing systems in vivo(4) and in vitro(5) for analysing RNA editing, its machinery still remains to be identified in higher plants. Here we introduce a genetic approach and report the discovery of a gene responsible for the specific RNA editing event in the chloroplast.