Recoverin as a paraneoplastic antigen in lung cancer: the occurrence of anti-recoverin autoantibodies in sera and recoverin in tumors
Recoverin as a paraneoplastic antigen in lung cancer: the occurrence of anti-recoverin autoantibodies in sera and recoverin in tumors
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DOI：
10.1016/j.lungcan.2003.10.006
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发表时间：
2004-05-01
期刊：
影响因子：
5.3
通讯作者：
Philippov, PP
中科院分区：
文献类型：
--
作者：
Bazhin, AV;Savchenko, MS;Philippov, PP
我们用重组回收素作为抗原，用免疫印迹法检测了279例小细胞肺癌（SCLC，99例）、非小细胞肺癌（NSCLC，44例）和非恶性肺部疾病（86例）患者的血清样本以及50例健康供体的血清。在15例SCLC患者（15%的病例）和9例NSCLC患者（约20%的病例）的血清中检测到针对恢复素（抗Rc）的自身抗体。非恶性肺疾病患者中仅2例抗Rc阳性，而健康人中未见白色阳性。44例小细胞肺癌和40例非小细胞肺癌石蜡切片的免疫组化显示30例（68%）和34例（85%）小细胞肺癌和非小细胞肺癌石蜡切片呈恢复阳性反应。尽管特异性高（98%），但敏感性低（低于20%），不允许将血清抗Rc视为有价值的肺癌标志物。然而，考虑到肺肿瘤中异常表达的高发生率，这种PNA可以被认为是肺癌免疫治疗的潜在靶点。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
Using immunoblotting with recombinant recoverin as an antigen, we have examined 279 serum samples from individuals with small cell lung carcinoma (SCLC, 99 patients), non-small cell lung carcinoma (NSCLC, 44 patients), and non-malignant pulmonary disorders (86 patients) as welt as sera from 50 healthy donors. Autoantibodies against recoverin (anti-Rc) were detected in sera from 15 patients with SCLC (15% of cases) and from 9 patients with NSCLC (about 20% of cases). Only two anti-Rc positive cases were detected in patients with non-malignant pulmonary disorders, white no such cases were found in healthy individuals. Immunohistochemical investigation of paraffin sections of 44 SCLC and 40 NSCLC tumors revealed recoverin-positive reaction in 30 SCLC (68%) and 34 NSCLC (85%) sections. Despite the high specificity (98%), the low sensitivity (Less than 20%) does not allow serum anti-Rc to be considered as a valuable marker of lung cancer. However, taking into account the high occurrence of aberrant expression of recoverin in lung tumors, this PNA could be considered as a potential target for immunotherapy of lung cancer. (C) 2003 Elsevier Ireland Ltd. All rights reserved.