Radar is required for the establishment of vascular integrity in the zebrafish
Radar is required for the establishment of vascular integrity in the zebrafish
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DOI：
10.1006/dbio.2002.0794
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发表时间：
2002-11-01
影响因子：
2.7
通讯作者：
Crosier, PS
中科院分区：
文献类型：
--
作者：
Hall, CJ;Flores, MVC;Crosier, PS
完整的血管网络的精确组装对于脊椎动物胚胎的存活至关重要。然而，原始内皮细胞形成能够向发育中的组织提供氧气和营养的成熟血管的过程仍然不完全清楚。在这里，我们提出了gdf 6的斑马鱼直系同源物之一Radar在建立斑马鱼胚胎躯干血管系统完整性方面的作用。我们表明，雷达表达适当放置，在空间和时间上，执行这样的角色。雷达的成绩单中检测到的hypochord和原肠内胚层。这些组织紧密地位于躯干中发育中的轴向血管的侧面，并且先前已经涉及血管发育的调节。基于吗啉代的靶向基因敲低产生了雷达特异性功能丧失的斑马鱼模型。这些胚胎显示正常的血管形成和循环的开始。然而，到发育的第2天，轴向脉管系统的完整性受到损害，整个发育中的躯干出现血管扩张和循环短路。我们表明，这种异常的血管发展是特定的雷达基因产物的减少。这些结果表明，雷达是参与信号通路所需的建立完整的轴向血管在斑马鱼的发展。(C)2002 Elsevier Science（美国）。
The precise assembly of an integrated network of blood vessels is essential for the survival of vertebrate embryos. However, the processes by which primitive endothelial cells form mature vessels capable of supplying oxygen and nutrients to developing tissues remain incompletely understood. Here, we propose a role for Radar, one of the zebrafish orthologues of gdf6, in establishing integrity of the trunk vasculature in zebrafish embryos. We show that radar expression is appropriately placed, both spatially and temporally, to perform such a role. Transcripts for radar are detected in the hypochord and the primitive gut endoderm. These tissues intimately flank developing axial vessels in the trunk and have been previously implicated in the regulation of vascular development. Morpholino-based targeted gene knock-down has generated a Radar-specific loss-of-function zebrafish model. These embryos display normal initiation of vascular patterning and commencement of circulation. However, by day 2 of development, the integrity of the axial vasculature is compromised with hemorrhages and circulation short-circuits throughout the developing trunk. We show that this aberrant vascular development is specific to a reduction of the radar gene product. These results suggest that Radar is involved in a signaling pathway required for establishing the integrity of the axial vessels during zebrafish development. (C) 2002 Elsevier Science (USA).