Cytolytic replication of echoviruses in colon cancer cell lines.
Cytolytic replication of echoviruses in colon cancer cell lines.
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DOI：
10.1186/1743-422x-8-473
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发表时间：
2011-10-14
期刊：
影响因子：
4.8
通讯作者：
Lindberg AM
中科院分区：
文献类型：
--
作者：
Israelsson S;Jonsson N;Gullberg M;Lindberg AM
结直肠癌是世界上最常见的癌症之一，近50%的患者死于结直肠癌。尽管在手术和其他补充治疗方面取得了进展，但仍然需要新的更有效的治疗方法。溶瘤病毒疗法，即通过病毒感染治愈癌症，是寻找新的和改进的治疗方法的一个有前途的领域。我们研究了几种临床少见的低致病力ECHO病毒的溶瘤潜能。ECHO病毒是肠道病毒属的一员，属于短小航病科。用人肠道病毒B型Echo病毒12、15、17、26和29感染6种结肠癌细胞系Caco-2、HT29、LoVo、SW480、SW620和T84，观察其细胞病变效应和病毒复制效果。在HT29细胞培养的球体中也进行了感染性测试。ECHO病毒12、17、26和29在几乎所有细胞系中有效复制，被认为具有高度的细胞溶解能力。在人工肿瘤(球体)中进一步评价了这四种病毒的感染性，发现ECHO病毒12、17和26容易感染球体。我们通过比较五种低致病力Echo病毒在六种结肠癌细胞系和人工肿瘤中的杀伤能力，发现Echo病毒12、17和26具有潜在的抗结肠癌溶瘤作用。
Colorectal cancer is one of the most common cancers in the world, killing nearly 50% of patients afflicted. Though progress is being made within surgery and other complementary treatments, there is still need for new and more effective treatments. Oncolytic virotherapy, meaning that a cancer is cured by viral infection, is a promising field for finding new and improved treatments. We have investigated the oncolytic potential of several low-pathogenic echoviruses with rare clinical occurrence. Echoviruses are members of the enterovirus genus within the family Picornaviridae. Six colon cancer cell lines (CaCo-2, HT29, LoVo, SW480, SW620 and T84) were infected by the human enterovirus B species echovirus 12, 15, 17, 26 and 29, and cytopathic effects as well as viral replication efficacy were investigated. Infectivity was also tested in spheroids grown from HT29 cells. Echovirus 12, 17, 26 and 29 replicated efficiently in almost all cell lines and were considered highly cytolytic. The infectivity of these four viruses was further evaluated in artificial tumors (spheroids), where it was found that echovirus 12, 17 and 26 easily infected the spheroids. We have found that echovirus 12, 17 and 26 have potential as oncolytic agents against colon cancer, by comparing the cytolytic capacity of five low-pathogenic echoviruses in six colon cancer cell lines and in artificial tumors.