ERIC-PCR identification of the spread of airborne Escherichia coli in pig houses.
ERIC-PCR identification of the spread of airborne Escherichia coli in pig houses.
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DOI：
10.1016/j.scitotenv.2009.12.019
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
W. Yuan;T. Chai;Z. Miao
中科院分区：
文献类型：
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作者：
W. Yuan;T. Chai;Z. Miao
为了解猪舍内微生物气溶胶的扩散情况，以大肠杆菌（E. coli）为指标，分别在猪舍上风向10、50米和下风向10、50、100、200和400米的不同位置采集4个猪舍及其周围空气中的大肠杆菌，并计算每个采样点的浓度。此外，收集猪粪便分离大肠杆菌。采用ERIC-PCR （Enterobacterial repeat Intergenic Consensus-Polymerase Chain Reaction，肠杆菌重复基因间一致聚合酶链反应）技术对分离得到的大肠杆菌DNA进行扩增，并用NTSYS-pc （Version 2.10）软件对扩增结果进行分析，以确定分离得到的大肠杆菌的相似性。结果表明：4个猪舍室内（21 ~ 35cfu）空气中大肠杆菌浓度显著高于上风向和下风向（P<0.05），而下风向间差异不显著（P<0.05）；ERIC-PCR结果还显示，52.4%的粪便大肠杆菌（4家分别为2/ 4,50%;2/ 4,50%;3/ 6,50%;4/ 7,57.1%）与室内空气中分离的大肠杆菌相同，10、50、100和200m处的下风空气中分离的大肠杆菌大部分（50%,21/42）与室内空气或粪便中分离的大肠杆菌相似度超过90%。由此可见，猪舍内微生物气溶胶具有向周围扩散的能力，应采取有效措施控制和减少微生物气溶胶的扩散。
To understand the spread of microbial aerosols in pig houses, with Escherichia coli (E. coli) as indicator, the airborne E. coli in 4 pig houses and their surroundings at different points 10, 50m upwind and 10, 50, 100, 200 and 400m downwind respectively from the pig houses were collected, and the concentrations were calculated at each sampling point. Furthermore, the feces of pigs were collected to separate E. coli. The ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction) technology was used to amplify the isolated E. coli DNA samples, then the amplified results were analyzed by NTSYS-pc (Version 2.10) to identify the similarity of isolated E. coli. The results showed that the airborne E. coli concentrations in indoor air of the 4 pig houses (21–35CFUm−3air) were much higher than those in upwind and downwind air (P<0.05), but there were no significant differences (P>0.05) at downwind distances. The ERIC-PCR results also showed that 52.4% of the fecal E. coli (four houses being respectively 2/4, 50%; 2/4, 50%; 3/6, 50%; 4/7, 57.1%) were identical to the indoor airborne E. coli isolates, and there was more than 90% similarity between the majority of E. coli (50%, 21/42) isolated from downwind air at 10, 50, 100 and 200m and those from indoor air or feces. It could be concluded that the aerosols in pig houses can spread to the surroundings, and thus effective measures should be taken to control and minimize the spread of microbial aerosols.