GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability.
GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability.
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GZFLW 通过促进 VPS53 蛋白稳定性诱导异位子宫内膜基质细胞凋亡。
DOI：
10.1155/2018/1293630
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
熊安稳
中科院分区：
文献类型：
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作者：
熊安稳
子宫内膜异位症仍然是妇产科的一大难题。桂枝茯苓丸最初用于治疗妇科疾病，但其治疗子宫内膜异位症的分子机制尚不清楚。为探讨桂枝茯苓丸治疗子宫内膜异位症的分子机制，采用iTRAQ（isobaric tags for relative and absolute quantification）蛋白质组学方法，对1例子宫内膜异位症患者的人子宫内膜间质细胞（Y14）进行体外研究。结果表明，桂枝五味汤能抑制Y14细胞增殖，促进细胞凋亡。通过iTRAQ偶联LC-MS/MS预测差异表达蛋白VPS 53（coloral protein sorting 53 homolog），并通过western blot进一步鉴定。此外，桂枝五味汤通过促进VPS 53蛋白的稳定而诱导VPS 53蛋白的表达。我们的研究结果强调了VPS 53在妇科中的新作用，并提供了一种有效的治疗子宫内膜异位症的策略。
Endometriosis is still a major problem in obstetrics and gynecology. While GZFLW (Gui Zhi Fu Ling Wan) has been originally used for treating gynecological diseases, however, the molecular mechanism that GZFLW acts on endometriosis is not clear. To investigate the molecular mechanism that GZFLW plays role on endometriosis, iTRAQ (isobaric tags for relative and absolute quantification) proteomics and human endometrial stromal cells (Y14) obtained from a patient with endometriosis were used in in vitro study. Our results demonstrated that GZFLW decreased Y14 cells proliferation while increased cells apoptosis. The differential expression protein VPS53 (Vacuolar protein sorting 53 homolog) was predicted by iTRAQ coupled LC-MS/MS and further identified by western blot. Besides, GZFLW induced VPS53 protein level by promoting its stabilization. Our findings highlight a novel role for VPS53 in gynecology and provide a potent therapeutic strategy against endometriosis.