STIMULATION OF INSULIN-SECRETION REVEALS HETEROGENEITY OF PANCREATIC B-CELLS INVIVO
STIMULATION OF INSULIN-SECRETION REVEALS HETEROGENEITY OF PANCREATIC B-CELLS INVIVO
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DOI：
10.1172/jci113045
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发表时间：
1987-07-01
影响因子：
15.9
通讯作者：
ORCI, L
中科院分区：
文献类型：
--
作者：
STEFAN, Y;MEDA, P;ORCI, L
我们研究了免疫荧光和超微结构的变化，胰岛素产生B细胞的中心和周围的胰岛在体内刺激期间，葡萄糖和格列本脲。葡萄糖刺激1.5小时后，在大鼠脾脏胰腺的胰岛中检测到胰岛素免疫染色减少。相比之下，免疫荧光变化变得明显，从十二指肠胰腺胰岛后，血糖> 3小时。在这两种情况下，免疫标记的中央B细胞减少之前，外周B细胞。格列本脲在体内刺激胰岛素分泌后观察到类似的变化。在超微结构水平上，高血糖使B细胞分泌颗粒体密度降低，粗面内质网和高尔基体密度增加。这些变化也检测到较早的中央比在外周B细胞和较早的脾脏比在十二指肠胰岛。数据显示B细胞在体内形成异质群体。
We examined the immunofluorescence and ultrastructural changes of insulin-producing B cells in the center and at the periphery of islets of Langerhans during in vivo stimulation by glucose and glibenclamide. A decreased insulin immunostaining was detected in islets from the splenic rat pancreas after 1.5 h of glucose stimulation. By contrast, immunofluorescence changes became apparent in islets from the duodenal pancreas only after > 3h of hyperglycemia. In both cases, the immunolabeling of central B cells decreased before that of peripheral B cells. Similar changes were seen following in vivo stimulation of insulin secretion by glibenclamide. At the ultrastructural level, hyperglycemia decreased the volume density of B cell secretory granules and increased that of rough endoplasmic reticulum and Golgi apparatus. These changes were also detected earlier in central than in peripheral B cells and earlier in splenic than in duodenal islets. The data show that B cells form a heterogeneous population in vivo.