Platelet Adhesion to Collagen is Inhibited by 5’ -Adenosine Diphosphate but Unaffected by Cell Shape
Platelet Adhesion to Collagen is Inhibited by 5’ -Adenosine Diphosphate but Unaffected by Cell Shape
复制标题
血小板与胶原蛋白的粘附受到 5-二磷酸腺苷的抑制，但不受细胞形状的影响
DOI：
10.1055/s-0039-1687237
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发表时间：
1979
影响因子：
6.7
通讯作者：
M. Frojmovic
中科院分区：
文献类型：
--
作者：
F. Meyer;Z. Weisman;M. Frojmovic
本文研究了ADP对兔血小板与胶原粘附的影响。在ADP浓度足以使PRP悬浮液中的血小板发生形状变化（>10- 8 M）时，观察到结合减少。然而，形状变化本身并不涉及。ADP对血小板形状和血小板粘附作用的时间依赖性不相关。如果防止形状变化，例如用1 μM PGE 1或固定剂处理，仍然会发生粘附力降低。洗涤后的血小板制剂，尽管被完全改变形状，也坚持不太好，在ADP的存在下。如所预期的，其形状在没有ADP参与的情况下（例如通过冷暴露处理）改变的血小板显示出与胶原的正常结合。ADP与血小板本身的结合是不够的，因为只有在结合发生后一段时间才能看到与胶原蛋白的粘附力降低。在ADP之前加入AMP可阻断ADP对血小板粘附的影响，但在ADP之后加入则无此作用。发生的时间依赖性事件可能是局部的，因为在固定血小板中观察到血小板粘附减少。这不太可能是ADP转化为ATP的结果。虽然ATP确实抑制粘附，但它仅在高得多的浓度下才能抑制粘附，并且仅在与ADP类似的滞后期之后才能抑制粘附。我们的研究结果表明，一些代理商报告，以减少血小板粘附胶原蛋白可能会这样做，使ADP从血小板释放。表面形状和化学
The effect of ADP on the adhesion of rabbit platelets to collagen from unstirred suspensions has been investigated. Reduced binding was seen at ADP concentrations sufficient for platelets in PRP suspensions to undergo shape change (>10-8M). However, shape change per se was not involved. The time dependence of the effect of ADP on platelet shape and on platelet adhesion did not correlate. Also reduced adhesion still occurred if shape change was prevented e.g. by treatment with 1 µM PGE1 or with fixatives. Washed platelet preparations in spite of being fully shape-changed also adhered less well in the presence of ADP. As expected platelets whose shape was changed without ADP being involved, e.g. by a cold exposure treatment, displayed normal binding to collagen. ADP binding to the platelet per se is not sufficient, since reduced adhesion to collagen is seen only some time after binding has taken place. Also AMP blocked the effect of ADP on platelet adhesion if added before but not after ADP. The time dependent event that occurs is likely to be local since reduced platelet adhesion was seen with fixed platelets. It is unlikely to result from the conversion of ADP to ATP. Although ATP does inhibit adhesion, it does so only at much higher concentrations and then only after a lag period similar to that seen with ADP. Our findings imply that some of the agents reported to reduce platelet adhesion to collagen may do so by causing ADP to be released from the platelet. Surface shape and chemistry