GROWTH CONE GUIDANCE BY SUBSTRATE-BOUND LAMININ PATHWAYS IS CORRELATED WITH NEURON-TO-PATHWAY ADHESIVITY
GROWTH CONE GUIDANCE BY SUBSTRATE-BOUND LAMININ PATHWAYS IS CORRELATED WITH NEURON-TO-PATHWAY ADHESIVITY
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DOI：
10.1016/0012-1606(88)90235-7
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发表时间：
1988-03-01
影响因子：
2.7
通讯作者：
LETOURNEAU, PC
中科院分区：
文献类型：
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作者：
HAMMARBACK, JA;MCCARTHY, JB;LETOURNEAU, PC
通过Hammarback等人的方法制备的底物结合的层粘连蛋白途径[J. A. Hammarback，S. L.棕榈湖T. Furcht和P.C. Letourneau（1985年）。J. Neurosci，Res. 13，213-220]引导外周神经系统神经突（分离的背根神经节和交感神经节）和中枢神经系统神经突（分离的脊髓和脑）。使用延时视频显微镜观察通过7-至10-μ m宽的层粘连蛋白途径对单个生长锥的引导。纤连蛋白途径，用于层粘连蛋白途径的方法产生的，不指导神经突。层粘连蛋白途径的指导作用进行了量化，并发现与层粘连蛋白的浓度最初施加到基板。层粘连蛋白的浓度最初适用于基层也与背根神经节（DRG）神经元的粘附性增加的层粘连蛋白构成的途径相对于紫外线照射的层粘连蛋白的边界的途径。层粘连蛋白通路的引导作用被抗层粘连蛋白抗体阻断或被层粘连蛋白阻断，但不被抗纤连蛋白抗体阻断。这项研究表明，层粘连蛋白的DRG神经突起的指导发生在生长锥的方式cosistent与差异神经元-基底层粘附性的指导假设。
Substrate-bound laminin pathways prepared by the method of Hammarback et al. [J. A. Hammarback, S. L. Palm, L. T. Furcht, and P. C. Letourneau (1985). J. Neurosci, Res. 13, 213-220] guided peripheral nervous system neurites (dissociated dorsal root ganglia and sympathetic ganglia) and central nervous system neurites (dissociated spinal cord and brain). Guidance of individual growth cones by 7- to 10-.mu.m-wide laminin pathways was observed using time-lapse video microscopy. Fibronectin pathways, produced by the method used for laminin pathways, did not guide neurites. The guidance effect of laminin pathways was quantified and found to correlate with the concentration of laminin initially applied to the substratum. The concentration of laminin initially applied to the substratum also correlated with increased adhesivity of dorsal root ganglia (DRG) neurons to laminin constituting the pathways relative to uv-irradiated laminin that borders the pathways. The guidance effect of laminin pathways was blocked by anti-laminin antibodies or by laminin but not by anti-fibronectin antibodies. This study demonstrates that guidance of DRG neurites by laminin occurs at the growth cone in a manner cosistent with the hypothesis of guidance by differential neuron-to-substratum adhesivity.