COR27 and COR28 Are Novel Regulators of the COP1-HY5 Regulatory Hub and Photomorphogenesis in Arabidopsis
COR27 and COR28 Are Novel Regulators of the COP1-HY5 Regulatory Hub and Photomorphogenesis in Arabidopsis
复制标题
COR27 和 COR28 是拟南芥中 COP1-HY5 调节中心和光形态发生的新型调节因子。
DOI：
10.1105/tpc.20.00195
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发表时间：
2020-10-01
期刊：
影响因子：
11.6
通讯作者：
Liu, Hongtao
中科院分区：
文献类型：
--
作者：
Li, Xu;Liu, Cuicui;Liu, Hongtao
COR 27和COR 28是COP 1-HY 5调控中心中的关键调节子，通过调节HY 5的活性来确保黑暗条件下的光形态发生生长和光照条件下的光形态发生发育。已知光和低温调节冷调节（COR）基因COR 27和COR 28的表达，其影响生物钟、抗冻性和开花时间。蓝光稳定了COR 27和COR 28蛋白，但其潜在机制尚不清楚。因此，我们进行了酵母双杂交筛选，使用COR 27-和COR 28作为诱饵，并确定E3泛素连接酶组成光形态发生1（COP 1）作为相互作用。COR 27和COR 28与COP 1发生物理相互作用，COP 1反过来又负责它们在黑暗中的降解。此外，COR 27和COR 28促进下胚轴伸长，并在拟南芥（Arabidopsis thaliana）中作为光形态建成的负调节因子。全基因组基因表达分析表明，HY 5，COR 27和COR 28共调节许多共同的基因。COR 27直接与HY 5相互作用，并与HY 5靶基因HY 5和PIF 4的启动子结合，然后与HY 5一起调节它们的转录。我们的结果表明，COR 27和COR 28作为COP 1-HY 5调节中心的关键调节子，通过与HY 5一起调节HY 5靶基因的转录，以确保黑暗中适当的skotomorphogenic生长和光照下的光形态发育。
COR27 and COR28 act as key regulators in the COP1-HY5 regulatory hub by regulating HY5 activity to ensure proper skotomorphogenic growth in the dark and photomorphogenic development in the light.Plants have evolved sensitive signaling systems to fine-tune photomorphogenesis in response to changing light environments. Light and low temperatures are known to regulate the expression of the COLD REGULATED (COR) genes COR27 and COR28, which influence the circadian clock, freezing tolerance, and flowering time. Blue light stabilizes the COR27 and COR28 proteins, but the underlying mechanism is unknown. We therefore performed a yeast two-hybrid screen using COR27- and COR28 as bait and identified the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) as an interactor. COR27 and COR28 physically interact with COP1, which is in turn responsible for their degradation in the dark. Furthermore, COR27 and COR28 promote hypocotyl elongation and act as negative regulators of photomorphogenesis in Arabidopsis (Arabidopsis thaliana). Genome-wide gene expression analysis showed that HY5, COR27, and COR28 co-regulate many common genes. COR27 interacts directly with HY5 and associates with the promoters of the HY5 target genes HY5 and PIF4, then regulates their transcription together with HY5. Our results demonstrate that COR27 and COR28 act as key regulators in the COP1-HY5 regulatory hub, by regulating the transcription of HY5 target genes together with HY5 to ensure proper skotomorphogenic growth in the dark and photomorphogenic development in the light.