The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features
The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features
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DOI：
10.1073/pnas.0711093105
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发表时间：
2008-02-12
影响因子：
11.1
通讯作者：
Gottschalk, Gerhard
中科院分区：
文献类型：
--
作者：
Seedorf, Henning;Fricke, W. Florian;Gottschalk, Gerhard
克氏梭菌在梭菌中是独特的;它以乙醇和乙酸作为唯一的能源厌氧生长。发酵产物是丁酸、己酸和H-2。我们在这里报告的基因组序列C。kluyveri，这揭示了新的见解，这种良好的研究有机体的代谢能力。膜结合的能量转换NADH：铁氧还蛋白氧化还原酶（RnfCDGEAB）和细胞质丁酰辅酶A脱氢酶复合物（Bcd/EtfAB）将巴豆酰辅酶A还原为丁酰辅酶A与铁氧还蛋白的还原偶联，代表了厌氧生物中新的能量保存模块。NAD依赖性乙醇脱氢酶和NAD（P）依赖性乙醛脱氢酶的基因位于微区室蛋白基因的旁边，这表明这两种酶在大分子复合物中分离在一起，形成了类似羧基体的结构。严格厌氧菌C. Kluyveri具有三组预测编码聚酮化合物/非核糖体肽合成酶杂合体的基因和一组编码非核糖体肽合成酶的基因。后者被预测催化合成一个新的铁载体，这是在缺铁的生长条件下形成的。
Clostridium kluyveri is unique among the clostridia; it grows anaerobically on ethanol and acetate as sole energy sources. Fermentation products are butyrate, caproate, and H-2. We report here the genome sequence of C. kluyveri, which revealed new insights into the metabolic capabilities of this well studied organism. A membrane-bound energy-converting NADH:ferredoxin oxidoreductase (RnfCDGEAB) and a cytoplasmic butyryl-CoA dehydrogenase complex (Bcd/EtfAB) coupling the reduction of crotonyl-CoA to butyryl-CoA with the reduction of ferredoxin represent a new energy-conserving module in anaerobes. The genes for NAD-dependent ethanol dehydrogenase and NAD(P)-dependent acetaldehyde dehydrogenase are located next to genes for microcompartment proteins, suggesting that the two enzymes, which are isolated together in a macromolecular complex, form a carboxysome-like structure. Unique for a strict anaerobe, C. kluyveri harbors three sets of genes predicted to encode for polyketide/nonribosomal peptide synthetase hybrides and one set for a nonribosomal peptide synthetase. The latter is predicted to catalyze the synthesis of a new siderophore, which is formed under iron-deficient growth conditions.