Insecticide susceptibility of natural populations of Anopheles coluzzii and Anopheles gambiae (sensu stricto) from Okyereko irrigation site, Ghana, West Africa
Insecticide susceptibility of natural populations of Anopheles coluzzii and Anopheles gambiae (sensu stricto) from Okyereko irrigation site, Ghana, West Africa
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DOI：
10.1186/s13071-016-1462-0
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发表时间：
2016-03-31
影响因子：
3.2
通讯作者：
Diclaro, Joseph W., II
中科院分区：
文献类型：
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作者：
Chabi, Joseph;Baidoo, Philip K.;Diclaro, Joseph W., II
背景：在撒哈拉以南非洲国家，疟疾媒介中杀虫剂耐药性的日益蔓延已得到充分记录。灌溉对增加病媒抵抗力的影响了解甚少，这对成功和合乎道德地实施粮食安全政策至关重要。本研究调查了大鲵对杀虫剂的抗性状况。从Okyereko的灌溉水稻区收集的冈比亚（s.l.）蚊子，Okyereko是加纳中部地区一个灌溉项目计划中约有42公顷灌溉农田的一个村庄。该地区通常使用大量杀虫剂、除草剂和化肥来提高水稻的年产量。方法：采集F-1子代蚊幼虫，测定成虫数量。采用WHO药敏试验和PCR方法分别鉴定耐药状况、等位基因和基因型。结果：冈比亚种群对DDT和拟除虫菊酯类杀虫剂具有高度抗性，可能与代谢机制有关，包括P450和GST酶以及P-gp活性的升高。人口主要由安人组成。Coluzzii标本（超过96%）KDR和ace-1频率分别为0.9%和0.2%。结论：本研究为黄麻对杀虫剂的抗性研究提供了新的信息。来自Okyereko的冈比亚（s.l）蚊子，这可能有助于该地区病媒控制规划的决策。
Background: The increasing spread of insecticide resistance in malaria vectors has been well documented across sub-Saharan Africa countries. The influence of irrigation on increasing vector resistance is poorly understood, and is critical to successful and ethical implementation of food security policies. This study investigated the insecticide resistance status of An. gambiae (s.l.) mosquitoes collected from the irrigated rice area of Okyereko, a village containing about 42 hectares of irrigated field within an irrigation project plan in the Central Region of Ghana. Large amounts of insecticides, herbicides and fertilizers are commonly used in the area to boost the annual production of the rice.Methods: Mosquito larvae were collected and adults were assayed from the F-1 progeny. The resistance status, allele and genotype were characterized using WHO susceptibility testing and PCR methods respectively.Results: The An. gambiae (s.l.) populations from Okyereko are highly resistant to DDT and pyrethroid insecticides, with possible involvement of metabolic mechanisms including the elevation of P450 and GST enzyme as well as P-gp activity. The population was mostly composed of An. coluzzii specimens (more than 96 %) with kdr and ace-1 frequencies of 0.9 and 0.2 %, respectively.Conclusion: This study brings additional information on insecticide resistance and the characterization of An. gambiae (s.l.) mosquitoes from Okyereko, which can be helpful in decision making for vector control programmes in the region.