Role of Olfactorily Responsive Neurons in the Right Dorsal Habenula-Ventral Interpeduncular Nucleus Pathway in Food-Seeking Behaviors of Larval Zebrafish
Role of Olfactorily Responsive Neurons in the Right Dorsal Habenula-Ventral Interpeduncular Nucleus Pathway in Food-Seeking Behaviors of Larval Zebrafish
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DOI：
10.1016/j.neuroscience.2019.01.057
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发表时间：
2019-04-15
期刊：
影响因子：
3.3
通讯作者：
Zhang, Bai-bing
中科院分区：
文献类型：
--
作者：
Chen, Wei-yu;Peng, Xiao-lan;Zhang, Bai-bing
缰核(Hb)在情绪相关行为中起重要作用。Hb除了接受边缘系统和基底节的输入外，还接受多种感觉模式的输入。斑马鱼Hb神经元的感觉反应是不对称的：左侧背侧Hb和右侧背侧Hb(DHb)分别优先对视觉和嗅觉刺激作出反应，暗示左右dHb的功能不同。虽然左侧dHb(L-dHb)的视觉反应与光偏好行为有关，但右侧dHb(R-dHb)的嗅觉反应的意义仍未得到充分研究。据报道，R-dHb可以阻断胆盐的先天吸引力。然而，考虑到R-dHb对各种气味的反应，研究R-dHb在其他嗅觉行为中的作用是有意义的，特别是对动物生存至关重要的寻找食物。在这里，我们利用活体全细胞记录和钙成像，首次表征了Hb神经元的食物提取物诱发的反应。反应神经元优先分布于R-而不是L-dHb，表现为开-型反应(类似87%)或关-型反应(类似13%)。有趣的是，这种从右到左的嗅觉反应不对称在Hb下游的主要靶点脚间核(IPN)中转化为腹向背侧模式。结合行为学分析，我们进一步发现R-dHb及其相应的下游腹侧IPN(V-IPN)的遗传功能障碍或损害削弱了游泳活动与觅食相关的增加。因此，我们的研究表明，R-dHb对V-IPN通路的不对称嗅觉反应在斑马鱼幼体的觅食行为中起着重要的作用。(C)2019 IBRO。爱思唯尔出版有限公司版权所有。
The habenula (Hb) plays important roles in emotion-related behaviors. Besides receiving inputs from the limbic system and basal ganglia, Hb also gets inputs from multiple sensory modalities. Sensory responses of Hb neurons in zebrafish are asymmetrical: the left dorsal Hb and right dorsal Hb (dHb) preferentially respond to visual and olfactory stimuli, respectively, implying different functions of the left and right dHb. While visual responses of the left dHb (L-dHb) have been implicated in light-preference behavior, the significance of olfactory responses of the right dHb (R-dHb) remains under-examined. It was reported that the R-dHb can gate innate attraction to a bile salt. However, considering a broad range of odors that R-dHb respond to, it is of interest to examine the role of R-dHb in other olfactory behaviors, especially food seeking, which is essential for animals' survival. Here, using in vivo whole-cell recording and calcium imaging, we first characterized food extract-evoked responses of Hb neurons. Responsive neurons preferentially locate in the R- but not L-dHb and exhibit either ON- (similar to 87%) or OFF-type responses (similar to 13%). Interestingly, this right-to-left asymmetry of olfactory responses converts into a ventral-to-dorsal pattern in the interpeduncular nucleus (IPN), a main downstream target of Hb. Combining behavior assay, we further found that genetic dysfunction or lesion of the R-dHb and its corresponding downstream ventral IPN (V-IPN) impair the food seeking-associated increase of swimming activity. Thus, our study indicates that the asymmetrical olfactory response in the R-dHb to V-IPN pathway plays an important role in food-seeking behavior of zebrafish larvae. (C) 2019 IBRO. Published by Elsevier Ltd. AU rights reserved.