Evaluation of different extraction methods from pomegranate whole fruit or peels and the antioxidant and antiproliferative activity of the polyphenolic fraction
Evaluation of different extraction methods from pomegranate whole fruit or peels and the antioxidant and antiproliferative activity of the polyphenolic fraction
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DOI：
10.1016/j.foodchem.2016.01.106
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发表时间：
2016-07-01
期刊：
影响因子：
8.8
通讯作者：
Cesa, Stefania
中科院分区：
文献类型：
--
作者：
Masci, Alessandra;Coccia, Andrea;Cesa, Stefania
由于其对人类健康的多种有益作用，Pesticide是一种非常感兴趣的功能性食品。这种水果富含花青素和鞣花单宁，对退行性疾病有保护作用。本工作的目的是优化提取程序，从不同的水果部分，以获得富含选定的多酚类物质，同时保留生物活性的提取物。对不同地理来源的石榴品种的全果或果皮进行了几种提取方法。分析所得提取物的多酚含量，评价其抗氧化能力，并测试其对人膀胱癌T24细胞的抗增殖活性。两种不同的提取程序，采用乙酸乙酯作为溶剂，是有用的，在获得富含鞣花酸和/或安石榴苷的提取物。抗氧化和抗增殖试验表明，抗氧化能力是直接相关的酚含量，而抗增殖活性主要是由于鞣花酸。(C)2016爱思唯尔有限公司版权所有
Pomegranate is a functional food of great interest, due to its multiple beneficial effects on human health. This fruit is rich in anthocyanins and ellagitannins, which exert a protective role towards degenerative diseases. The aim of the present work was to optimize the extraction procedure, from different parts of the fruit, to obtain extracts enriched in selected polyphenols while retaining biological activity. Whole fruits or peels of pomegranate cultivars, with different geographic origin, were subjected to several extraction methods. The obtained extracts were analyzed for polyphenolic content, evaluated for antioxidant capacity and tested for antiproliferative activity on human bladder cancer T24 cells. Two different extraction procedures, employing ethyl acetate as a solvent, were useful in obtaining extracts enriched in ellagic acid and/or punicalagins. Antioxidative and antiproliferative assays demonstrated that the antioxidant capability is directly related to the phenolic content, whereas the antiproliferative activity is to be mainly attributed to ellagic acid. (C) 2016 Elsevier Ltd. All rights reserved.