Effect of chemical ablation of myenteric neurons on neurotransmitter levels in the rat jejunum.
Effect of chemical ablation of myenteric neurons on neurotransmitter levels in the rat jejunum.
复制标题
肌间神经元化学消融对大鼠空肠神经递质水平的影响。
DOI：
10.1016/0016-5085(87)90126-0
复制
发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Bass,P
中科院分区：
文献类型：
--
作者：
Dahl,JL;Bloom,DD;Epstein,ML;Fox,DA;Bass,P
我们量化了大鼠空肠中神经递质的变化，其中肌间神经元通过浆膜应用苯扎氯铵而被消融。苯扎氯铵治疗后 2 天内，胆碱乙酰转移酶（胆碱能神经元的特异性标记物）的活性降低了 40%，每厘米空肠长度的血管活性肠肽的量降低了 25%。到 15 天时，其水平与对照组相当，而到肌间神经元消融后 45 天，治疗组织中的水平是对照组的两倍。相比之下，我们观察到苯扎氯铵治疗后早期空肠每厘米长度的亮氨酸脑啡肽含量没有减少，尽管到 45 天时，空肠治疗段中这种神经递质的水平是对照组的两倍多。肌间神经元消融后 15、30 和 45 天还观察到肌肉厚度和组织重量显着增加。因此，我们观察到，作为对大鼠空肠中肌间神经元化学消融的反应，平滑肌层增厚，并且肠道中幸存的神经元元件产生的某些神经递质代偿性增加。
We have quantified neurotransmitter changes in the rat jejunum in which the myenteric neurons were ablated by serosal application of benzalkonium chloride. Within 2 days after benzalkonium chloride treatment, there was a 40% reduction in the activity of choline acetyltransferase, a specific marker for cholinergic neurons, and a 25% reduction in the amount of vasoactive intestinal peptide per centimeter length of jejunum. By 15 days, levels were comparable to those in control segments, and by 45 days after myenteric neuronal ablation, levels in treated tissues were twice those in controls. In contrast, we observed no reduction in the amount of leucine-enkephalin per centimeter length of jejunum at early times after benzalkonium chloride treatment, although by 45 days, levels of this neurotransmitter in treated segments of jejunum were more than twice those in controls. Significant increases in muscle thickness and tissue weight were also observed at 15, 30, and 45 days after myenteric neuronal ablation. Thus we have observed that in response to chemical ablation of myenteric neurons in the rat jejunum, there is a thickening of the smooth muscle layers and a compensatory increase in the production of certain neurotransmitters by the surviving neuronal elements in the gut.