RETT-SYNDROME - 3-D CONFOCAL MICROSCOPY OF CORTICAL PYRAMIDAL DENDRITES AND AFFERENTS
RETT-SYNDROME - 3-D CONFOCAL MICROSCOPY OF CORTICAL PYRAMIDAL DENDRITES AND AFFERENTS
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DOI：
10.1097/00001756-199407000-00025
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发表时间：
1994-07-21
期刊：
影响因子：
1.7
通讯作者：
DAHLSTROM, A
中科院分区：
文献类型：
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作者：
BELICHENKO, PV;OLDFORS, A;DAHLSTROM, A
对3例瑞特综合征患者和6例对照病例（额叶、颞叶和运动皮质区）的尸检材料进行了研究。通过荧光黄微量注射使每个标本中的100 - 200个锥体神经元和非锥体神经元可视化。使用VoxelView/GT程序进行三维重建和旋转。观察到不同皮质中锥体神经元的三维树突结构存在多种类型的异常。II - III层的一些锥体细胞树突数量低于正常。不同皮质区域中锥体结构正常出现的特化现象缺失。发现树突棘数量减少以及区域缺失（“裸露”区域）。通过羰花青染料（DiI）示踪法显示皮质传入纤维分布广泛。与在癫痫中观察到的情况相反，病理学解剖诊断（PAD）未显示微小发育不良。
AUTOPSY material from three patients with Rett syndrome and six control cases (frontal, temporal and motor cortical areas) were studied. Pyramidal and non-pyramidal neurones, 100-200 in each specimen, were visualized by Lucifer Yellow microinjection. 3-D reconstructions and rotations were computed using the VoxelView/GT program. Various types of abnormalities in 3-D dendritic architecture of pyramidal neurones in different cortices were observed. Some pyramidal cells in layers II-III had a subnormal number of dendrites. The normally occurring specializations in pyramidal architecture in different cortical areas were absent. Decreased numbers and regional loss ('naked' areas) of dendritic spines were found. Widely distributed cortical afferents were shown by the carbocyanine dye (DiI) tracing method. PAD showed no microdysgenesis, in contrast to the observations in epilepsy.