A study on producing monoclonal antibody with one diagnostic marker screened electrophoretically from the urine of individuals infected with Schistosoma japonicum
A study on producing monoclonal antibody with one diagnostic marker screened electrophoretically from the urine of individuals infected with Schistosoma japonicum
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DOI：
10.1016/s0732-8893(00)00207-8
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发表时间：
2000-12-01
影响因子：
2.9
通讯作者：
Guan, XH
中科院分区：
文献类型：
--
作者：
Zhu, CL;Tian, HS;Guan, XH
一种诊断标志物（一种30 kDa的糖蛋白）来自感染了S.以日本血吸虫为抗原制备单克隆抗体。获得了两株单抗，命名为NP 56和NP 54。单克隆抗体NP 56具有较好的免疫反应性，其免疫球蛋白同种型为IgG（2b），能与虫卵可溶性抗原SEA和成虫抗原AWA反应，并能在虫卵中表达毛蚴。日本的以NP 56为探针进行间接ELISA检测，阳性尿样（EPG中位数为69）的敏感性和特异性在原始尿样中分别为50%和80%，在浓缩尿样中分别为90%和100%。上述结果表明，用已建立的方法从感染者的代谢产物中筛选诊断标志物制备McAb是可行的，McAb-NP_（56）对慢性感染者和轻度感染者具有潜在的诊断价值。山茱萸(C)2000 Elsevier Science Inc. All rights reserved.
One diagnostic marker (a glycoprotein with 30kDa) from the urine of individuals infected with S. japonicum was screened with electrophoresis, and used as antigen to produce McAbs. Two lines of McAbs, named NP56 and NP54, were obtained. McAb NP56 was of better immunoreactivity and its immunoglobulin isotype was IgG(2b), McAb NP56 could react with SEA (soluble egg antigen) and AWA (adult worm antigen) and express an miracidia in eggs of S. japonicum. When determined with NP56 as a probe in indirect ELISA, the sensitivity and specificity of positive urine samples (EPG median 69) was 50% and 80% in original urine samples, and 90% and 100% in concentrated urine samples, respectively. The results above indicated that with established means it is feasible to produce McAb with the diagnostic marker screened electrophoretically from the metabolic product of the individuals infected with this pathogen, and that McAb-NP56 is of potential value in the diagnosis of chronic cases and individuals with light infection with S. japonicum. (C) 2000 Elsevier Science Inc. All rights reserved.