Effects of silk fibroin in murine dry eye.
Effects of silk fibroin in murine dry eye.
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DOI：
10.1038/srep44364
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发表时间：
2017-03-10
影响因子：
4.6
通讯作者：
Yang J
中科院分区：
文献类型：
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作者：
Kim CE;Lee JH;Yeon YK;Park CH;Yang J
该研究旨在研究丝素蛋白在干眼症小鼠模型中的作用。使用超过12周龄的NOD. B10. H2 b小鼠，将其暴露于30-40%的环境湿度并注射氢溴酸东莨菪碱10天，开发实验性干眼小鼠模型。通过滴注磷酸盐缓冲盐水或丝素蛋白来测量泪液产生和角膜不规则性评分。采用苏木精-伊红或过碘酸希夫染色法观察角膜或结膜脱离及杯状细胞密度。免疫组化法检测泪腺组织中炎性标志物的表达。蚕丝组泪液分泌增加，角膜光滑度改善。蚕丝组角膜上皮细胞和结膜杯状细胞恢复正常。蚕丝组泪腺炎症因子的表达受到抑制。这些结果表明丝素蛋白改善了干眼症小鼠模型中的角膜、结膜和泪腺。这些发现表明，丝素蛋白在干眼症的实验模型中具有抗炎作用。
The study aimed to investigate the effects of silk fibroin in a mouse model of dry eye. The experimental dry eye mouse model was developed using more than twelve-weeks-old NOD.B10.H2b mice exposing them to 30–40% ambient humidity and injecting them with scopolamine hydrobromide for 10 days. Tear production and corneal irregularity score were measured by the instillation of phosphate buffered saline or silk fibroin. Corneal detachment and conjunctival goblet cell density were observed by hematoxylin and eosin or periodic acid Schiff staining in the cornea or conjunctiva. The expression of inflammatory markers was detected by immunohistochemistry in the lacrimal gland. The silk group tear production was increased, and corneal smoothness was improved. The corneal epithelial cells and conjunctival goblet cells were recovered in the silk groups. The expression of inflammatory factors was inhibited in the lacrimal gland of the silk group. These results show that silk fibroin improved the cornea, conjunctiva, and lacrimal gland in the mouse model of dry eye. These findings suggest that silk fibroin has anti-inflammatory effects in the experimental models of dry eye.