VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN IS ANCHORED IN THE VIRAL MEMBRANE BY A HYDROPHOBIC DOMAIN NEAR THE COOH TERMINUS
VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN IS ANCHORED IN THE VIRAL MEMBRANE BY A HYDROPHOBIC DOMAIN NEAR THE COOH TERMINUS
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DOI：
10.1073/pnas.77.7.3884
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
LING, NC
中科院分区：
文献类型：
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作者：
ROSE, JK;WELCH, WJ;LING, NC
测定了水疱性口炎病毒（VSV）糖蛋白(G)的cooh末端和nh2末端氨基酸序列。从一个克隆的DNA插入物的完整序列中推断出122个cooh末端氨基酸序列，该插入物携带来自gmrna 3'端的470个核苷酸。多肽的这一部分包括位于病毒粒子内部并跨越病毒粒子脂质双分子层的G结构域。这似乎很清楚，因为在彻底的蛋白水解消化后，与病毒粒子膜相关的G片段的部分氨基酸序列可以明确地定位在预测序列中。该预测序列包含一个不间断的疏水结构域，从COOH端开始49个氨基酸，结束30个氨基酸。这个区域可能横跨脂质双分子层。29个氨基酸的cooh末端部分含有很高比例的碱性残基，并存在于病毒粒子内。因此，VSV - G蛋白的cooh末端在结构上类似于糖蛋白，糖蛋白是一种红细胞膜蛋白。这里所示的病毒膜中G的结构可能与其他病毒糖蛋白相似，尽管没有在任何其他情况下进行直接测试。从相邻的M蛋白基因延伸到G蛋白基因的RNA基因组上的DNA引物序列，推断出53个氨基酸的nh2末端G蛋白序列，其中包括16个氨基酸的先导序列。该序列证实，扩展和纠正了先前报道的该区域的2个部分氨基酸序列。
The COOH-terminal and NH2-terminal amino acid sequences of the vesicular stomatitis virus (VSV) glycoprotein (G) were determined. A sequence of 122 COOH-terminal amino acids was deduced from the complete sequence of a cloned DNA insert carrying 470 nucleotides derived from the 3'' end of the G mRNA. This portion of the polypeptide includes the domains of G that reside inside the virion and span the lipid bilayer of the virion. This seems clear because a partial amino acid sequence of a fragment of G that remains associated with the membrane of the virion after exhaustive proteolytic digestions can be located unambiguously in the predicted sequence. This predicted sequence contains an uninterrupted hydrophobic domain beginning 49 amino acids and ending 30 amino acids from the COOH terminus. This region presumably spans the lipid bilayer. The COOH-terminal portion of 29 amino acids contains a high proportion of basic residues and resides inside the virion. The COOH-terminal portion of the VSV G protein therefore resembles in structure that of glycophorin, an erythrocyte membrane protein well characterized previously. The configuration of G in the viral membrane demonstrated here is probably similar for other viral glycoproteins, although this was not tested as directly in any other case. From the sequence of a DNA primer extended on the RNA genome from the adjacent M protein gene into the G protein gene, an NH2-terminal G protein sequence of 53 amino acids was deduced, including the leader sequence of 16 amino acids. The sequence confirms, extends and corrects 2 partial amino acid sequences reported for this region previously.