Behaviour of nucleotides and oligonucleotides in potentiometric HPLC detection
Behaviour of nucleotides and oligonucleotides in potentiometric HPLC detection
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电位 HPLC 检测中核苷酸和寡核苷酸的行为
DOI：
10.1016/j.aca.2005.06.049
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发表时间：
2005
影响因子：
6.2
通讯作者：
L. Nagels
中科院分区：
文献类型：
--
作者：
Y. Bao;Joseph Everaert;M. Pietraszkiewicz;O. Pietraszkiewicz;H. Bohets;H. Geise;B. Peng;L. Nagels
研究了单核苷酸(UMP、AMP、UDP、ADP、CTP、UTP、GTP、ATP)和寡核苷酸(d(T)12-185‘OH、21-聚体、33-聚体和60-聚体的混合物)的电位法高效液相色谱检测方法。采用镀丝电极。涂层为液膜型，含有PVC、DOS和合成的大环胺、豆荚和尿素受体。基于这些受体的电极对三磷酸核苷酸和寡核苷酸有非常灵敏的响应。寡核苷酸的摩尔响应与其摩尔质量有关。反相柱用磷酸盐缓冲液、乙酸三乙胺(TEAA)和乙腈梯度洗脱。讨论了这些高电荷态离子的灵敏电位响应。
Potentiometric HPLC detection was studied of mononucleotides (UMP, AMP, UDP, ADP, CTP, UTP, GTP, ATP) and oligonucleotides (a synthetic mixture d(T)12–185′OH, a mixed 21-mer, 33-mer, and 60-mer). Coated-wire electrodes were used. The coatings were of the liquid membrane type containing PVC, DOS and synthetic macrocyclic amine- and podand ureum receptors. Electrodes based on these receptors gave very sensitive responses to triphosphate nucleotides and to oligonucleotides. The molar response of the oligonucleotides was related to their molar mass. The HPLC system consisted of a reversed phase column eluted with a phosphate buffer, triethylammoniumacetate (TEAA), and an acetonitrile gradient. The sensitive potentiometric response of these highly charged ions is discussed.
影响因子：
7.4
作者：
FU, B;BAKKER, E;MEYERHOFF, ME
通讯作者：
MEYERHOFF, ME