Hydrogen peroxide spraying alleviates drought stress in soybean plants
Hydrogen peroxide spraying alleviates drought stress in soybean plants
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DOI：
10.1016/j.jplph.2011.02.003
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发表时间：
2011-09-01
影响因子：
4.3
通讯作者：
Zheng, Shao-Hui
中科院分区：
文献类型：
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作者：
Ishibashi, Yushi;Yamaguchi, Haruka;Zheng, Shao-Hui
为了确定外源过氧化氢(H_2O_2)对干旱胁迫的影响，我们研究了大豆叶片喷施H_2O_2是否会缓解干旱胁迫的症状。与喷洒蒸馏水(DW)的叶片相比，喷洒过氧化氢的叶片处理延缓了干旱胁迫引起的叶片枯萎。此外，经过氧化氢处理的干旱胁迫叶片的相对含水量高于经DW处理的叶片。因此，我们分析了干旱胁迫下喷施H_2O_2对叶片光合作用参数和与保水有关的寡糖生物合成的影响。在干旱胁迫条件下，过氧化氢处理的叶片净光合速率和气孔导度均高于DW处理的叶片。与OW喷施不同，喷施H_2O_2立即导致D-肌醇3-磷酸合成酶2(GmMIPS2)和半乳糖醇合成酶(GOL)的mRNA水平增加，这两种酶编码低聚糖生物合成的关键酶，已知有助于植物抗旱。此外，过氧化氢处理的叶片中肌醇和半乳糖醇的含量高于DW处理的叶片。这些结果表明，喷施H_2O_2能够使大豆植株通过维持叶片含水量来避免干旱胁迫，并且这种保水是由于促进了低聚糖的生物合成而不是通过气孔的快速关闭造成的。(C)2011年爱思唯尔股份有限公司。版权所有。
To ascertain the effect of exogenously applied hydrogen peroxide (H2O2) on drought stress, we examined whether the spraying of soybean leaves with H2O2 would alleviate the symptoms of drought stress. Pretreatment by spraying leaves with H2O2 delayed foliar wilting caused by drought stress compared to leaves sprayed with distilled water (DW). Additionally, the relative water content of drought-stressed leaves pre-treated with H2O2 was higher than that of leaves pre-treated with DW. Therefore, we analyzed the effect of H2O2 spraying on photosynthetic parameters and on the biosynthesis of oligosaccharides related to water retention in leaves during drought stress. Under conditions of drought stress, the net photosynthetic rate and stomatal conductance of leaves pre-treated with H2O2 were higher than those of leaves pre-treated with DW. In contrast to OW spraying, H2O2 spraying immediately caused an increase in the mRNA levels of D-myo-inositol 3-phosphate synthase 2 (GmMIPS2) and galactinol synthase (GolS), which encode key enzymes for the biosynthesis of oligosaccharides known to help plants tolerate drought stress. In addition, the levels of myo-inositol and galactinol were higher in H2O2-treated leaves than in DW-treated leaves. These results indicated that H2O2 spraying enabled the soybean plant to avoid drought stress through the maintenance of leaf water content, and that this water retention was caused by the promotion of oligosaccharide biosynthesis rather than by rapid stomatal closure. (C) 2011 Elsevier GmbH. All rights reserved.