Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris
Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris
复制标题
DOI：
10.1263/jbb.92.585
复制
发表时间：
2001-12-01
影响因子：
2.8
通讯作者：
Meagher, MM
中科院分区：
文献类型：
--
作者：
Inan, M;Meagher, MM
毕赤酵母在甘油或葡萄糖和甲醇的混合物中生长遵循双重生长，C-1利用的酶被抑制。因此，这些碳源不能与甲醇混合使用来同时培养pasteroris和利用酶诱导C-1，特别是在缺乏aox的pasteroris的摇瓶培养中。当以甲醇作为诱导剂时，丙氨酸、山梨醇、甘露醇和海藻糖等碳源均未抑制β -gal的产生。结果表明，丙氨酸、山梨醇、甘露醇和海藻糖均可作为酵母的唯一碳源和能量来源，但海藻糖的加倍时间很长。在含有海藻糖、丙氨酸、山梨醇和甘露醇的培养基中，以甲醇（0.5%）作为诱导剂生长的Mut（-）菌株与在含甲醇培养基中生长的Mut（+）菌株表达的β -gal量相同或更高。
The growth of Pichia pastoris in a mixture of either glycerol or glucose and methanol follows a diauxic growth, with C-1 utilizing enzymes being repressed. Therefore, these carbon sources can not be used as a mixture with methanol to simultaneously grow P. pastoris and induce C-1 utilizing enzymes, especially in a shake flask cultures of AOX-deficient P. pastoris. Among the alternative carbon sources tested, alanine, sorbitol, mannitol and trehalose, did not repress beta-gal production when methanol was used as an inducer in mut(-) strain of P. pastoris. Our results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on trehalose was very long. Mut(-) strains growing in media containing trehalose, alanine, sorbitol and mannitol with methanol (0.5%) as an inducing agent expressed as much or higher amount of beta-gal as compared to the mut(+) growing in methanol containing media.