Human Breast Cancer Cell Lines as Models of Growth Regulation and Disease Progression
Human Breast Cancer Cell Lines as Models of Growth Regulation and Disease Progression
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DOI：
10.1007/bf02096306
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发表时间：
1996-01-01
影响因子：
2.5
通讯作者：
Ethier, Stephen P.
中科院分区：
文献类型：
--
作者：
Ethier, Stephen P.
30多年来，从患者样品中的人类乳腺癌细胞的常规隔离和培养一直是乳腺癌细胞生物学家的目标。尽管在该地区进行了广泛的工作以及许多人类乳腺癌细胞系的发展，但导致永生的乳腺癌细胞系的患者样品的比例仍然令人失望。已经建立的大多数人乳腺癌细胞系都是孤立的，并且在较差的培养条件下不断地生长。这些细胞系对人类乳腺癌细胞中雌激素受体生物学的研究很有用，可在鉴定由乳腺癌细胞合成的生长因子以及这些细胞中存在的癌基因和肿瘤抑制基因的遗传改变的表征。最近，组织培养方法有所改善，从而导致特定谱系的常规人类乳腺上皮细胞的培养能力，这导致了新的人类乳腺癌细胞系的发展。在相似的培养条件下隔离和培养正常和肿瘤的人类乳腺上皮细胞的能力已大大改善了这些模型，并导致鉴定出人类乳腺癌细胞的细胞表型改变。
The routine isolation and culture of human breast cancer cells from patient samples has been a goal of breast cancer cell biologists for over 30 years. Despite extensive work in this area and the development of many human breast cancer cell lines, the proportion of patient samples that give rise to immortalized breast cancer cell lines is still disappointingly low. The majority of human breast cancer cell lines that have been established were isolated many years ago and have been grown continuously under poorly defined culture conditions. These cell lines have been useful for studies of the estrogen receptor biology in human breast cancer cells, in identifying growth factors synthesized by breast cancer cells, and for the characterization of genetic alterations in oncogenes and tumor suppressor genes present in these cells. More recently, tissue culture methods have improved, resulting in the ability to culture routinely normal human mammary epithelial cells of specific lineages and this has resulted in the development of new human breast cancer cell lines. The ability to isolate and culture normal and neoplastic human mammary epithelial cells under similar culture conditions has improved these models dramatically and has resulted in the identification of altered cellular phenotypes of human breast cancer cells.