Fructose 2,6-bisphosphate 2 years after its discovery.
Fructose 2,6-bisphosphate 2 years after its discovery.
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果糖 2,6-二磷酸发现两年后。
DOI：
10.1042/bj2060001
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
VanSchaftingen,E
中科院分区：
文献类型：
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作者：
Hers,HG;VanSchaftingen,E
Fru-2，6-P2的发现胰高血糖素对肝脏PFK的影响1979年，四组研究人员（Castano等人，1979; Clarke等人，1979; Kagimoto & Uyeda，1979; Pilkis等人，1979）报道了在胰高血糖素存在下孵育细胞后，在肝细胞提取物中测量的PFK的动力学性质被改变。激素的作用是降低酶对Fru-6-P的亲和力，增强ATP的抑制作用。第一组和第三组研究者报告，这些效应在凝胶过滤和酶解纯化后保持稳定。它们被认为是PFK被环AMP依赖性蛋白激酶磷酸化的结果（Castano等人，1979; Kagimoto和Uyeda，1979，1980; Claus等人，1980年a）。相反，当在该实验室中重新研究相同的问题时（货车Schaftingen等人，所用缩写：Fru-1，6-P2，果糖1，6-二磷酸; Fru-2，6-P2，果糖2，6-二磷酸; Fru-6-P，果糖6-磷酸; Glc-1，6-P2，葡萄糖1，6-二磷酸; FBPase或FBPase 1，果糖1，6-二磷酸酶; FBPase 2，果糖-2，6-二磷酸酶; PFK或PFK 1，6-磷酸果糖-1-激酶; PFK 2，6-磷酸果糖-2-激酶。
The discovery ofFru-2, 6-P2 The effect ofglucagon on liver PFK In 1979, four groups of investigators (Castano et al., 1979; Clarke et al., 1979; Kagimoto & Uyeda, 1979; Pilkis et al., 1979) reported that the kinetic properties of PFK, measured in extracts of hepatocytes, were modified after incubation of the cells in the presence of glucagon. The hormonal action was to decrease the affinity of the enzyme for Fru-6-P and to increase the inhibitionby ATP. These effects were reported by the first and the third groups of investigators to be stable upon gel ifitration and purification of the enzymic prepara-tion. They were believed to be the consequence of a phosphorylation of PFK by cyclic AMP-dependent protein kinase (Castano et al., 1979; Kagimoto& Uyeda, 1979, 1980; Claus et al., 1980a). In contrast, when the same problem was re-investigated in this laboratory (Van Schaftingen et al., Abbreviations used: Fru-1, 6-P2, fructose 1, 6-bisphos-phate; Fru-2, 6-P2, fructose 2, 6-bisphosphate; Fru-6-P, fructose 6-phosphate; Glc-1, 6-P2, glucose 1, 6-bisphosphate; FBPase or FBPase 1, fructose 1, 6-bisphosphat-ase; FBPase 2, fructose-2, 6-bisphosphatase; PFK or PFK 1, 6-phosphofructo-1-kinase; PFK 2, 6-phosphofructo-2-kinase.