Upregulation of endothelial receptor for oxidized LDL (LOX-1) by oxidized LDL and implications in apoptosis of human coronary artery endothelial cells - Evidence from use of antisense LOX-1 mRNA and chemical inhibitors
Upregulation of endothelial receptor for oxidized LDL (LOX-1) by oxidized LDL and implications in apoptosis of human coronary artery endothelial cells - Evidence from use of antisense LOX-1 mRNA and chemical inhibitors
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DOI：
10.1161/01.atv.20.4.1116
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发表时间：
2000-04-01
影响因子：
8.7
通讯作者：
Mehta, JL
中科院分区：
文献类型：
--
作者：
Li, DY;Mehta, JL
氧化低密度脂蛋白 (LOX-1) 的特定凝集素样内皮受体与单核细胞/巨噬细胞中的清道夫受体不同，已被鉴定并克隆。在这项研究中，我们检查了氧化低密度脂蛋白（ox-LDL）对LOX-I的调节，并确定了LOX-1在ox-LDL诱导培养的人冠状动脉内皮细胞（HCAEC）凋亡中的作用。将 HCAEC 与 ox-LDL (40 μg/mL)（而非天然 LDL）一起孵育 24 小时，LOX-I 表达（mRRTA 和蛋白质）显着增加。将 HCAEC 与 LOX-I mRNA 的特异性反义（反义 LOX-1）预孵育 48 小时后，ox-LDL 介导的 LOX-I 上调受到抑制（P
A specific lectinlike endothelial receptor for oxidized low density lipoprotein (LOX-1), distinct from the scavenger receptor in monocytes/macrophages, has been identified and cloned. In this study, we examined the regulation of LOX-I by oxidized low density Lipoprotein (ox-LDL) and determined the role of LOX-1 in ox-LDL-indnced apoptosis of cultured human coronary artery endothelial cells (HCAECs). Incubation of HCAECs with ox-LDL (40 mu g/mL), but not native LDL, for 24 hours markedly increased LOX-I expression (mRRTA and protein). After 48 hours of preincubation of HCAECs with a specific antisense to LOX-I mRNA (antisense LOX-1), ox-LDL mediated upregulation of LOX-I was suppressed (P