3D-printed photoluminescent bioactive scaffolds with biomimetic elastomeric surface for enhanced bone tissue engineering
3D-printed photoluminescent bioactive scaffolds with biomimetic elastomeric surface for enhanced bone tissue engineering
复制标题
具有仿生弹性体表面的 3D 打印光致发光生物活性支架，用于增强骨组织工程
DOI：
10.1016/j.msec.2019.110153
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发表时间：
2020-01-01
影响因子：
7.9
通讯作者：
Lei, Bo
中科院分区：
文献类型：
--
作者：
Chen, Mi;Zhao, Fujian;Lei, Bo
3D打印多孔生物活性玻璃纳米颗粒支架（BGNS）具有良好的骨整合和骨再生能力，但其早期离子快速释放、脆性力学性能和功能缺失限制了其应用。本文通过在BGNS表面直接组装聚柠檬酸盐硅氧烷（PCS）制备了光致发光仿生弹性体BGNS （BGNS@PCS）。详细评价了BGNS@PCS的形态、力学行为、光致发光能力、离子释放、生物矿化活性、生物相容性和成骨性能。结果表明，与BGNS相比，BGNS@PCS具有优异的弹性和抗压强度。在BGNS@PCS中实现了硅和钙离子的可控释放，并观察到增强的生物矿化能力。此外，改性支架具有光致发光能力，在生物成像方面具有潜在的应用前景。BGNS@PCS能显著促进小鼠骨髓基质细胞（BMSCs）的附着、增殖和成骨分化。因此，BGNS@PCS具有弹性体表面、增强光致发光、控制离子释放和生物矿化、增强成骨活性等多功能特性，是骨组织再生的理想材料。本研究为硬组织再生仿生弹性生物陶瓷支架的设计提供了一种新的思路。
Three dimensional (3D) printed porous bioactive glass nanoparticles scaffolds (BGNS) exhibit excellent bone integration and bone regeneration capacities, but the early rapid ion release, brittle mechanical properties and lack of functions limit their application. In this work, photoluminescent biomimetic elastomeric BGNS were fabricated by directly assembling poly(citrate-siloxane) (PCS) on the surface of BGNS (BGNS@PCS). The morphologies, mechanical behavior, photoluminescent ability, ions release, biomineralization activity, biocompatibility and osteogenic properties of BGNS@PCS were evaluated in detail. The results indicated that BGNS@PCS presented superior elasticity and outstanding compressive strength compared with BGNS. The controlled release of the Si and Ca ions in BGNS@PCS was achieved and enhanced biomineralization ability was also observed. In addition, the modified scaffolds have the photoluminescent ability which has the potential application for bioimaging. BGNS@PCS could significantly promote cells attachment, proliferation and enhance osteogenic differentiation of mouse bone marrow stromal cells (BMSCs). Therefore, the BGNS@PCS with the multifunctional properties including elastomeric surface, enhanced photoluminescent, controlled ions release and biomineralization, reinforced osteogenic activity, would be a promising candidate for bone tissue regeneration. This study probably provides a novel strategy to design biomimetic elastomeric bioceramic scaffolds for hard tissue regeneration.