Tumour plasticity and extravascular circulation in ECV304 human bladder carcinoma cells.
Tumour plasticity and extravascular circulation in ECV304 human bladder carcinoma cells.
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发表时间：
2006
影响因子：
2
通讯作者：
A. Fujimoto;H. Onodera;A. Mori;S. Nagayama;Y. Yonenaga;T. Tachibana
中科院分区：
文献类型：
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作者：
A. Fujimoto;H. Onodera;A. Mori;S. Nagayama;Y. Yonenaga;T. Tachibana
背景血管生成拟态和马赛克血管的概念已被提出作为肿瘤新血管形成的新模式。然而，这些类型的新生血管的存在和意义仍然不清楚。材料和方法ECV304人膀胱癌细胞用于确定肿瘤细胞如何参与肿瘤新生血管形成。结果ECV304小鼠皮下移植瘤血管类型多样，包括血管生成血管、肿瘤细胞相关血管和细胞外基质网络。示踪剂实验证明了这些结构中的珠的灌注。ECV304细胞，培养在胶原蛋白I凝胶，形成管网络与几个内皮相关的标志物的表达。在ECV304细胞和人脐静脉内皮细胞共培养模型中，两种细胞协同形成芽或网络。结论ECV304细胞具有内皮细胞特性，具有与血管内皮细胞相似和协同作用的能力，有利于肿瘤微循环的获得。
BACKGROUND The concepts of vasculogenic mimicry and mosaic vessels have been proposed as novel modes of tumour neovascularisation. However, the presence and significance of these types of neovascularisation remain unclear. MATERIALS AND METHODS ECV304 human bladder carcinoma cells were used to determine how tumour cells take part in tumour neovascularisation. RESULTS Subcutaneous ECV304 xenografts in mice showed various vessel types, including angiogenic vessels, tumour cell-related vessels and extracellular matrix networks. A tracer experiment demonstrated perfusion of beads in these structures. ECV304 cells, cultured on collagen I gels, formed tube networks with expressions of several endothelial-related markers. In coculture models of ECV304 cells and human umbilical vein endothelial cells, the two cells collaborated to form sprouts or networks. CONCLUSION ECV304 cells possess an endothelial character which confers the ability to mimic and collaborate with vascular endothelial cells and facilitates the acquisition of tumour microcirculation.