Distribution of cells bearing receptors for a colony-stimulating factor (CSF-1) in murine tissues.
Distribution of cells bearing receptors for a colony-stimulating factor (CSF-1) in murine tissues.
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DOI：
10.1083/jcb.91.3.848
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发表时间：
1981-12
影响因子：
7.8
通讯作者：
Stanley, E R
中科院分区：
文献类型：
--
作者：
Byrne, P V;Guilbert, L J;Stanley, E R
CSF-1是集落刺激因子的一个亚类，其特异性刺激单核吞噬细胞的生长。我们使用125 I-CSF-1在0 ℃下与来自各种鼠组织的单细胞悬浮液结合，结合放射自显影，以确定结合细胞的频率、它们的身份和每个结合细胞的结合位点的数量。对于检查的所有组织，在2- 3 × 10（-10）M 125 I-CSF-1下，结合位点在2小时内达到饱和。结合是不可逆的，用5 × 10（-10）M CSF-1预孵育2 h几乎完全阻断。4.3%的骨髓细胞、7.5%的血液单个核细胞、2.4%的脾细胞、20.5%的腹腔细胞、11.8%的肺泡细胞和0.4%的淋巴结细胞显示125 I-CSF-1结合。四种形态学上可区分的细胞类型结合125 I-CSF-1：母细胞;核质面积比（N/C）大于1的单核细胞;具有锯齿状核的细胞;以及N/C小于或等于1的单核细胞。在血液粒细胞或胸腺细胞中未检测到CSF-1结合细胞。骨髓早幼粒细胞、中幼粒细胞、嗜酸性粒细胞、嗜酸性粒细胞、有核红细胞、无核红细胞和巨核细胞也未能结合。单个核细胞颗粒计数的频率分布是均匀的。相反，骨髓、脾、肺泡和腹膜细胞的细胞是异质的。血液或骨髓中的单核细胞（小细胞，具有缩进的核或具有大于1的N/C）被相对均匀地标记，每个细胞具有约3，000个结合位点。较大的结合细胞（例如，肺泡细胞）可能具有更高数量的受体。它的结论是，CSF-1结合仅限于单核吞噬细胞及其前体，它可以用来识别这一系列的成熟和不成熟的细胞。
CSF-1 is a subclass of the colony-stimulating factors that specifically stimulates the growth of mononuclear phagocytes. We used the binding of 125I-CSF-1 at 0 degrees C by single cell suspensions from various murine tissues, in conjunction with radioautography, to determine the frequency of binding cells, their identity, and the number of binding sites per binding cell. For all tissues examined, saturation of binding sites was achieved within 2 h at 2--3 x 10(-10) M 125I-CSF-1. The binding was irreversible and almost completely blocked by a 2 h preincubation with 5 x 10(-10) M CSF-1. 125I-CSF-1 binding was exhibited by 4.3% of bone marrow cells, 7.5% of blood mononuclear cells, 2.4% of spleen cells, 20.5% of peritoneal cells, 11.8% of pulmonary alveolar cells and 0.4% of lymph node cells. Four morphologically distinguishable cell types bound 125I-CSF-1: blast cells; mononuclear cells with a ratio of nuclear to cytoplasmic area (N/C) greater than 1; cells with indented nuclei; and mononuclear cells with N/C less than or equal to 1. No CSF-1 binding cells were detected among blood granulocytes or thymus cells. Bone marrow promyelocytes, myelocytes, neutrophilic granulocytes, eosinophilic granulocytes, nucleated erythroid cells, enucleated erythrocytes, and megakaryocytes also failed to bind. The frequency distribution of grain counts per cell for blood mononuclear cells was homogenous. In contrast, those for bone marrow, spleen, alveolar, and peritoneal cells were heterogeneous. The monocytes in blood or bone marrow (small cells, with either indented nuclei or with N/C greater than 1) were relatively uniformly labeled, possessing approximately 3,000 binding sites per cell. Larger binding cells (e.g., alveolar cells) may possess higher numbers of receptors. It is concluded that CSF-1 binding is restricted to mononuclear phagocytic cells and their precursors and that it can be used to identify both mature and immature cells of this series.