FACS-style detection for real-time cell viscoelastic cytometry.
FACS-style detection for real-time cell viscoelastic cytometry.
复制标题
用于实时细胞粘弹性细胞计数的 FACS 式检测。
DOI：
10.1039/c5ra24097b
复制
发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Marr,DWM
中科院分区：
文献类型：
--
作者：
Kasukurti,A;Eggleton,CD;Desai,SA;Marr,DWM
细胞机械特性已被确立为细胞活力和健康的无标记生物物理标志；然而，具有高通量、能够准确且非破坏性地测量这些特性的实时方法仍然广泛存在。如果没有适合荧光激活细胞分选仪 (FACS) 的标签，当前仍需要轻松实现跟踪细胞级机械特性的实时技术。利用调制光学力并通过此处介绍的低维 FACS 式检测方法实现，我们提出了一种能够实时连续测量的粘弹性细胞仪 (VC)。我们通过以~1 s−1 的速率跟踪化学修饰细胞群的高频细胞物理特性来证明这种方法的实用性，并在简单的理论模型的背景下解释观察结果。
Cell mechanical properties have been established as a label-free biophysical marker of cell viability and health; however, real-time methods with significant throughput for accurately and non-destructively measuring these properties remain widely unavailable. Without appropriate labels for use with fluorescence activated cell sorters (FACS), easily implemented real-time technology for tracking cell-level mechanical properties remains a current need. Employing modulated optical forces and enabled by a low-dimensional FACS-style detection method introduced here, we present a viscoelasticity cytometer (VC) capable of real-time and continuous measurements. We demonstrate the utility of this approach by tracking the high-frequency cell physical properties of populations of chemically-modified cells at rates of ∼1 s−1 and explain observations within the context of a simple theoretical model.