EFFECTS OF GROWTH FACTORS ON CORNEAL WOUND HEALING
EFFECTS OF GROWTH FACTORS ON CORNEAL WOUND HEALING
复制标题
生长因子对角膜伤口愈合的影响
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
S. Mackay
中科院分区：
文献类型：
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作者：
G. Schultz;N. Chegini;M. Grant;P. Khaw;S. Mackay
摘要眼表创伤的愈合是一个复杂的过程，涉及上皮细胞和基质细胞的迁移、有丝分裂和分化。内源性产生的肽生长因子，如表皮生长因子（EGF），转化生长因子α（TGF-α）或转化生长因子β（TGF-β）可能在自然伤口愈合过程中发挥关键作用。据报道，泪腺细胞合成并分泌EGF到泪液中，其可通过外分泌途径促进角膜上皮和基质损伤的愈合。EGF刺激培养的上皮细胞和基质成纤维细胞的DNA合成，刺激上皮细胞合成纤维连接蛋白，并对人上皮细胞和基质细胞具有趋化性。人角膜上皮细胞也合成TGF-α，其可能通过自分泌途径影响上皮细胞。TGF-β是人巩膜成纤维细胞培养物中赖氨酰氧化酶mRNA水平的有效诱导剂，可能是损伤后诱导角膜细胞外基质成分合成的最主要因素。用外源性肽生长因子治疗上皮损伤的眼表伤口也能加速兔和灵长类动物的愈合。用EGF、纤连蛋白（一种合成胶原酶抑制剂）和抑肽酶联合治疗由碱引起的严重眼表损伤可显著阻断溃疡形成并增强上皮再生。表皮生长因子局部治疗眼表伤口的临床试验表明，肽生长因子可能是一种有价值的辅助治疗眼表伤口。
Abstract Healing of ocular surface wounds is a complex process involving migration, mitosis, and differentiation of epithelial and stromal cells. Endogenously produced peptide growth factors such as epidermal growth factor (EGF), transforming growth factor alpha (TGF‐α), or transforming growth factor beta (TGF‐β) may play key roles in the natural wound healing process. Lacrimal gland cells were reported to synthesize and secrete EGF into tear fluid where it may enhance healing of corneal epithelial and stromal injuries by an exocrine pathway. EGF stimulated DNA synthesis of epithelial cells and stromal fibroblasts in culture, stimulated synthesis of fibronectin by epithelial cells and was chemotactic for human epithelial and stromal cells. Human corneal epithelial cells also synthesized TGF‐α which may influence epithelial cells by an autocrine pathway. TGF‐β, which is a potent in‐ducer of lysyl oxidase mRNA levels in cultures of human scleral fibroblasts, may be the factor most responsible for inducing synthesis of corneal extracellular matrix components after an injury. Treatment of epithelial injuries ocular surface wounds with exogenous peptide growth factors also accelerated healing in rabbits and primates. Treatment of severe ocular surface injuries caused by alkali with a combination of EGF, fibronectin, a synthetic collagenase inhibitor, and Aprotinin significantly blocked ulceration and enhanced epithelial regeneration. Clinical trials of topical treatment of EGF for ocular surface wounds suggest that peptide growth factors may be a valuable adjuvant for treatment of ocular surface wounds.
表皮生长因子对角膜伤口愈合的剂量依赖性影响。
影响因子：
4.4
作者：
Mathers,WD;Sherman,M;Fryczkowski,A;Jester,JV
通讯作者：
Jester,JV