Morphological and neurochemical characterization of electrophysiologically identified cells
Morphological and neurochemical characterization of electrophysiologically identified cells
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电生理学鉴定细胞的形态学和神经化学特征
DOI：
10.1007/978-1-0716-1522-5_24
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Kubota Y
中科院分区：
文献类型：
--
作者：
Hayashi-Takagi A;Kubota Y
现在众所周知，大脑中有许多神经元亚型。例如，在新皮层中发现每层中的锥体细胞的几个亚型和10个或更多的非锥体细胞亚型。它们在微电路中的活性和功能作用在每个细胞亚型中是不同的。因此，神经元亚型识别对于了解记录的神经元的功能作用非常重要，其生理放电特性被研究。神经元亚型在形态学和神经化学上不同，因此记录细胞的组织学和免疫组织化学染色促进了细胞识别。在这一章中，组织学组织制备方法，化学标记物鉴定，树突和轴突分支跟踪分析，并通过电子显微镜观察，包括块面扫描显微镜的生物胞素注射记录的细胞进行了描述。
It is now well known that there are many neuron subtypes in the brain. For instance, a few subtypes of pyramidal cell in each layer and 10 or more non-pyramidal cell subtypes are found in the neocortex. Their activity and functional role in the microcircuit are different among each cell subtype. Therefore, neuron subtype identification is very important to understand the functional role of the recorded neurons whose physiological firing properties are studied. Neuronal subtypes are morphologically and neurochemically distinct, so histological and immunohistochemical staining of the recorded cells promotes the cell identification. In this chapter, histological tissue preparation methods, for chemical marker identification, dendritic and axonal arborization tracing analysis, and observation by electron microscopy including block-face scanning microscopy of biocytin-injected recorded cells are described.