Inhibitory effect of MyoD on the proliferation of breast cancer cells
Inhibitory effect of MyoD on the proliferation of breast cancer cells
复制标题
MyoD对乳腺癌细胞增殖的抑制作用
DOI：
10.3892/ol.2016.4448
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发表时间：
2016-06-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Chi
中科院分区：
文献类型：
--
作者：
Cai, Changjing;Qin, Xiaoqun;Liu, Chi
骨骼肌淋巴管丰富，血供丰富，是肿瘤转移的少见部位。先前的研究表明，当骨骼肌损伤或癌变时，MyoD的分泌可能会增强。推测MyoD可能作为一种内源性细胞因子抑制癌细胞的增殖。为了验证该蛋白对肿瘤细胞增殖的可能影响，用包埋的Transwell平板对C2C12小鼠骨骼肌细胞和4T1小鼠乳腺癌细胞进行了共培养。共培养后，细胞周期分析显示C2C12肌肉细胞能够抑制乳腺癌细胞的增殖。随后，在C2C12细胞中沉默MyoD以评估其对4T1细胞增殖的影响。与MyoD沉默的细胞共培养后，5-乙炔-20-脱氧尿嘧啶核苷检测表明，MyoD沉默可阻止未转染的C2C12细胞对4T1细胞增殖的抑制。综上所述，这些结果表明MyoD抑制乳腺癌细胞的增殖，可能是一种肿瘤抑制因子。
Skeletal muscle is rich in lymphatic vessels, with an abundant blood supply, and it is an infrequent site of cancer metastasis. Previous studies have demonstrated that enhanced secretion of MyoD may occur when skeletal muscle is injured or becomes cancerous. It was hypothesized that MyoD may act as an endogenous cytokine to inhibit the proliferation of cancer cells. To verify the possible effect of this protein on tumor cell proliferation, C2C12 mouse skeletal muscle cells and 4T1 mouse breast cancer cells were co-cultured using embedded Transwell plates. Following co-culture, cell cycle analysis revealed that C2C12 muscle cells were able to inhibit the proliferation of the breast cancer cells. Subsequently, MyoD was silenced in C2C12 cells to assess its effect on 4T1 cell proliferation. Following co-culture with MyoD-silenced cells, a 5-ethynyl-20-deoxyuridine assay indicated that MyoD silencing prevented the reduction in proliferation of 4T1 cells induced by untransfected C2C12 cells. In summary, the results indicated that MyoD inhibits the proliferation of breast cancer cells and may be a tumor suppressor factor.