Computerized quantification of tissue vascularization using high-resolution slide scanning of whole tumor sections
Computerized quantification of tissue vascularization using high-resolution slide scanning of whole tumor sections
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DOI：
10.1177/002215540305100203
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发表时间：
2003-02-01
影响因子：
3.2
通讯作者：
McNamara, G
中科院分区：
文献类型：
--
作者：
Chantrain, CF;DeClerck, YA;McNamara, G
利用免疫组织化学技术进行微血管检测的组织血管化评估由于难以产生可重复的定量数据而受到限制。个体血管的区分和待分析显微场的选择仍然是两个主观性因素。在这项研究中，我们使用成像分析软件和高分辨率切片扫描仪测量了人类神经母细胞瘤和小鼠乳腺腺癌肿瘤全切片的cd31免疫染色内皮区域（EA）。影像分析软件为不同肿瘤的切片分析提供了客观标准。利用滑动扫描仪获得的整个肿瘤切片图像的标准构成了一种快速量化肿瘤血管化的方法。与先前描述的方法，即“热点”和“随机场”方法相比，我们的“全切片扫描”方法获得的EA测量结果具有更高的可重复性，“全切片扫描”方法的观察者间歧异率为8.6%，而“热点”方法和“随机场”方法的观察者间歧异率分别为42.2%和39.0%。同时采用全断面扫描法测量微血管密度，并提供血管分布和大小的附加数据。因此，该方法是一种具有时间效率和可重复性的肿瘤血管化定量方法。
Assessment of tissue vascularization using immunohistochemical techniques for microvessel detection has been limited by difficulties in generating reproducible quantitative data. The distinction of individual blood vessels and the selection of microscopic fields to be analyzed remain two factors of subjectivity. In this study, we used imaging analysis software and a high-resolution slide scanner for measurement of CD31-immunostained endothelial area (EA) in whole sections of human neuroblastoma xenograft and murine mammary adenocarcinoma tumors. Imaging analysis software provided objective criteria for analysis of sections of different tumors. The use of the criteria on images of entire tumor section acquired with the slide scanner constituted a rapid method to quantify tumor vascularization. Compared with previously described methods, the "hot spot" and the "random fields" methods, EA measurements obtained with our "whole section scanning" method were more reproducible with 8.6% interobserver disagreement for the :'whole section scanning" method vs 42.2% and 39.0% interobserver disagreement for the 'hot spot" method and the "random fields," respectively. Microvessel density was also measured with the whole section scanning method and provided additional data on the distribution and the size of the blood vessels. Therefore, this method constitutes a time efficient and reproducible method for quantification of tumor vascularization.