Presence of human epidermal growth factor in human cerebrospinal fluid.
Presence of human epidermal growth factor in human cerebrospinal fluid.
复制标题
人脑脊液中存在人表皮生长因子。
DOI：
10.1210/jcem-55-6-1174
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Shigeru Matsukura
中科院分区：
文献类型：
--
作者：
Yukio Hirata;M. Uchihashi;H. Nakajima;Takuo Fujita;Shigeru Matsukura
人表皮生长因子（gEGF），一种有效的刺激生长的许多组织的文化，已被分离出人尿，随后确定在许多人的生物体液。我们已经部分纯化和鉴定了人脑脊液（CSF）中的hEGF样物质，并测定了各种神经系统疾病患者CSF中免疫反应性（IR）hEGF的浓度。CSF样品产生的竞争性结合曲线在RIA和使用人胎盘膜的放射受体测定中似乎与标准hEGF平行。CSF提取物的Sephadex G-50凝胶排阻色谱显示与标准hEGF共洗脱的IR-hEGF的单峰。用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法测得CSF中hEGF样物质的表观摩尔质量为8000，等电聚焦法测得其pI约为4.5。垂体瘤、脑肿瘤和脊髓神经根病患者CSF中IR-hEGF的浓度显著高于对照组，而脑积水和脑脑膜炎患者CSF中IR-hEGF的水平与对照组无统计学差异。人CSF中存在hEGF样物质表明其可能在人神经组织的功能中发挥重要的生理作用，但没有提供其来源的任何证据。
Human epidermal growth factor (gEGF), a potent stimulator of the growth of many tissues in culture, has been isolated from human urine and subsequently identified in many human biological fluids. We have partially purified and characterized hEGF-like substance(s) from human cerebrospinal fluid (CSF) and determined the concentrations of immunoreactive (IR) hEGF in CSF obtained from patients with a variety of neurological diseases. Competitive binding curves generated by the CSF samples appeared to be parallel to standard hEGF both in RIA and radioreceptor assays using human placental membrane. Sephadex G-50 gel exclusion chromatography of the CSF extract revealed a single peak of IR-hEGF which coeluted with standard hEGF. The apparent mol wt of the CSF hEGF-like substances(s) was estimated to be 8000 by sodium dodecyl sulfate poplyacrylamide gel electrophoresis, and its approximate pI was 4.5 as determined by isoelectric focusing. The concentrations of IR-hEGF in CSF from patients with pituitary and brain tumors and radiculomyelopathy were significantly higher than those from control subjects, while neither patients with hydrocephalus nor encephalomeningitis had CSF IR-hEGF levels statistically different from the control subjects. The presence of hEGF-like substance(s) in human CSF suggests that it may play an important physiological role in the function of the human nervous tissues but does not provide any evidence of its source.