Immunohistochemical Localization of B7 Costimulating Molecules and Major Histocompatibility Complex Class II Antigen in Pulmonary Sarcoidosis
Immunohistochemical Localization of B7 Costimulating Molecules and Major Histocompatibility Complex Class II Antigen in Pulmonary Sarcoidosis
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肺结节病中 B7 共刺激分子和主要组织相容性复合物 II 类抗原的免疫组织化学定位
作者：
Y. Kaneko;K. Kuwano;R. Kunitake;M. Kawasaki;N. Hagimoto;H. Miyazaki;T. Maeyama;Takuo Tanaka;T. Matsuba;N. Hara
背景：结节病的肺泡巨噬细胞（AM）介导抗原诱导的T淋巴细胞增殖的能力增强。抗原呈递细胞不仅要向T淋巴细胞呈递带有MHC分子的抗原肽，还要表达B7共刺激分子，才能诱导有效的免疫应答。目的：探讨B7和MHC分子在结节病患者肺组织中的表达。方法：以结节病患者经支气管肺活检标本和单纯性肺结节肺叶切除术后获得的正常肺实质标本为对照，对B7-1、B7-2和MHCⅱ类抗原进行免疫组化检测。结果：15例结节病患者肉芽肿上皮样细胞中分别有14例（93.3%）、2例（13.3%）和9例（60.0%）表达B7-1、B7-2和MHCⅱ类抗原。在15例结节病患者中，AM中分别有14例（93.3%）、5例（33.3%）和12例（80.0%）表达。结节病上皮样细胞和AM中B7-1的表达均显著高于B7-2 （p < 0.01）。AM中B7-1、B7-2和MHC II类抗原阳性信号分别在15例对照中有9例（90%）、8例（80%）和8例（80%）。AM结节病患者B7-1阳性信号强度显著高于对照组（p < 0.05）， B7-2和MHCⅱ类抗原阳性信号强度不显著高于对照组（p < 0.05）。结论：这些结果提示结节病患者肉芽肿和AM的上皮样细胞具有辅助细胞的功能，并且在结节病中这些细胞的辅助功能由B7-2向B7-1转移。
Background: Alveolar macrophages (AM) of sarcoidosis have an enhanced capacity to mediate antigen-induced T lymphocyte proliferation. To induce an effective immune response, antigen-presenting cells have to not only present antigenic peptide with MHC molecules to T lymphocytes, but also express B7 costimulating molecules. Objective: The purpose of this study was to investigate the expression of B7 and MHC molecules in lung tissues from patients with sarcoidosis. Methods: We performed immunohistochemistry for B7-1, B7-2 and MHC class II antigens using transbronchial lung biopsy specimens obtained from patients with sarcoidosis and normal lung parenchyma obtained by lobectomy for solitary pulmonary nodule as controls. Results: B7-1, B7-2 and MHC class II antigen were expressed in epithelioid cells in granulomas in 14 (93.3%), 2 (13.3%) and 9 (60.0%) of 15 patients with sarcoidosis, respectively. These were also expressed in AM in 14 (93.3%), 5 (33.3%) and 12 (80.0%) of 15 patients with sarcoidosis, respectively. The positivitiy of B7-1 was significantly higher than that of B7-2 in both epithelioid cells and AM in sarcoidosis (p < 0.01). Positive signals for B7-1, B7-2 and MHC class II antigen were also found in AM in 9 (90%), 8 (80%) and 8 (80%) of 15 of controls, respectively. However, the intensity of positive signals for B7-1, but not B7-2 or MHC class II antigen in AM was significantly increased in sarcoidosis compared to controls (p < 0.05). Conclusions: These results suggested that epithelioid cells in granulomas and AM from patients with sarcoidosis had the capability to act as accessory cells and that the accessory function of these cells was shifted to B7-1 rather than B7-2 in sarcoidosis.
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来自结节病患者的支气管肺泡细胞表现出增强的抗原呈递。
DOI：
--
发表时间：
1985
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lem,VM;Lipscomb,MF;Weissler,JC;Nunez,G;Ball,EJ;Stastny,P;Toews,GB
通讯作者：
Toews,GB
影响因子：
56.9
作者：
LENSCHOW, DJ;ZENG, YJ;BLUESTONE, JA
通讯作者：
BLUESTONE, JA
人肺泡巨噬细胞无法刺激静息 T 细胞与抗原特异性 T 细胞巨噬细胞结合减少相关。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lyons,CR;Ball,EJ;Toews,GB;Weissler,JC;Stastny,P;Lipscomb,MF
通讯作者：
Lipscomb,MF
DOI：
10.1073/pnas.90.14.6586
发表时间：
1993-07-15
影响因子：
11.1
作者：
GIMMI, CD;FREEMAN, GJ;NADLER, LM
通讯作者：
NADLER, LM
人肺泡巨噬细胞对淋巴细胞增殖的调节。
DOI：
10.1164/arrd.1986.133.6.1091
发表时间：
1986
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Ettensohn,DB;Lalor,PA;RobertsJr,NJ
通讯作者：
RobertsJr,NJ